Abstract
Spatial variations regulation of expression of CuZn‐SOD and Mn‐SOD by shear stress in vascular tissue
The FASEB journal, Vol.20(5), pp.A1452-A1452
03/2006
DOI: 10.1096/fasebj.20.5.A1452-c
Abstract
Introduction
There are important spatial variations between shear stress and focal and eccentric atherosclerotic lesions. We tested whether pulsatile shear stress (PSS) vs. oscillatory shear stress (OSS) regulates SOD isoforms; namely, SOD1 (CuZn‐SOD), SOD2 (Mn‐SOD), and SOD3 (extracellular SOD), in bovine aortic endothelial cells (BAEC) and vascular smooth muscle cells (VSMC).
Methods
Confluent BAEC and VSMC monolayers were exposed to PSS at a mean shear stress (τave) of 23 dyn.cm−2 and a temporal gradient (∂τ/∂ t) at 71 dyn.cm. −2.sec−2; and OSS at τave= 0.02 ± 3 dyn.cm−2 in a dynamic parallel plate flow system for 4 hours. SOD‐mRNA isoforms were measured with real‐time RT‐PCR.
Results
a)BAEC (Fig. 1): SOD2 was significantly up‐regulated in BAEC in response to shear stress (P <0.05, n=3). PSS increased SOD2 expression by 10‐fold and OSS by 5‐fold. SOD1 was increased by 2‐fold in response to PSS, but remained unchanged in response to OSS. SOD3 did not respond to shear stress.
1
Endothelial SOD mRNA expression
b)VSMC (Fig. 2): SOD2 was up‐regulated in SMC in response to PSS by 2‐fold, but was down‐regulated in response to OSS (P < 0.05, n=3). SOD1 expressed the similar trend, but was statistically insignificant. SOD3 expression was statistically insignificant.
2
Smooth muscle SOD mRNA expression
Conclusion
Shear stress increased expression of SOD1 and ‐2 suggesting that spatial variations in shear stress may contribute to spatial differences in vascular oxidative stress.
Details
- Title: Subtitle
- Spatial variations regulation of expression of CuZn‐SOD and Mn‐SOD by shear stress in vascular tissue
- Creators
- Mahsa Rouhanizadeh - Biomedical EngineeringUniversity of Southern California1042 Downey Way Denney Research Center, (DRB) 398Los AngelesCA90089Tiantian C Lin - Biomedical EngineeringUniversity of Southern California1042 Downey Way Denney Research Center, (DRB) 398Los AngelesCA90089Yi Chu - University of IowaDonald Heistad - University of IowaTzung K Hsiai - University of Southern California
- Resource Type
- Abstract
- Publication Details
- The FASEB journal, Vol.20(5), pp.A1452-A1452
- Publisher
- Federation of American Societies for Experimental Biology
- DOI
- 10.1096/fasebj.20.5.A1452-c
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Number of pages
- 1
- Language
- English
- Date published
- 03/2006
- Academic Unit
- Cardiovascular Medicine; Internal Medicine; Neuroscience and Pharmacology
- Record Identifier
- 9984304742002771
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