Abstract
The dithiol antioxidant, bucillamine, protects against cardiac dysfunction and remodeling in a murine model of myocardial ischemia‐reperfusion injury
The FASEB journal, Vol.20(4), pp.A230-A230
03/2006
DOI: 10.1096/fasebj.20.4.A230-a
Abstract
Abstract only
It is well established that restoration of blood flow through occluded arteries improves outcome following myocardial infarction. Paradoxically, restoration of flow may offset the benefit of reoxygenation due to the production of reactive oxygen species (ROS) that can alter signal transduction and cause cellular damage. We thus tested the ability of the potent dithiol antioxidant, bucillamine, to protect against long-term ischemia-reperfusion (I/R) injury in a mouse model of myocardial infarction. Myocardial ischemia was achieved by transiently occluding the left anterior descending coronary artery for 30 minutes. Within 5 minutes of initiating reperfusion, saline or bucillamine was administered via tail vein injection. Drug treatment continued with daily subcutaneous injections for 4 weeks. Bucillamine was effective in partially preserving cardiac function following I/R injury, as determined by echocardiographic measurement of fractional shortening (saline: sham 53∀1%, I/R 35∀ 4%; bucillamine: sham 51∀3%, I/R 41∀4%; mean ∀ SE; p < 0.05, sham vs I/R, saline I/R vs bucillamine I/R). Furthermore, I/R induction of changes in hypertrophic gene expression (ANP, α- and β-myosin heavy chain, SERCA), as measured by ribonuclease protection assay, was also attenuated by bucillamine. We conclude that prolonged administration of a potent dithiol antioxidant preserves ventricular function and attenuates abnormal gene expression associated with pathologic cardiac remodeling.
Details
- Title: Subtitle
- The dithiol antioxidant, bucillamine, protects against cardiac dysfunction and remodeling in a murine model of myocardial ischemia‐reperfusion injury
- Creators
- S. Kelly Ambler - CardiologyUCHSC4200 E 9th AveDenverCO80262, DHMC777 Bannock StDenverCO80204Yvonne K. Hodges - CardiologyUCHSC4200 E 9th AveDenverCO80262Gayle M. Jones - CardiologyUCHSC4200 E 9th AveDenverCO80262Carlin S. Long - CardiologyUCHSC4200 E 9th AveDenverCO80262, DHMC777 Bannock StDenverCO80204Lawrence D. Horwitz - CardiologyUCHSC4200 E 9th AveDenverCO80262
- Resource Type
- Abstract
- Publication Details
- The FASEB journal, Vol.20(4), pp.A230-A230
- DOI
- 10.1096/fasebj.20.4.A230-a
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Language
- English
- Date published
- 03/2006
- Academic Unit
- Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984657737202771
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