Abstract
The effect of infectious and inflammatory stimuli on the synovium and resident synovial macrophages in a novel human explant model 9007
The Journal of immunology (1950), Vol.214(Supplement_1), vkaf2832472
11/01/2025
DOI: 10.1093/jimmun/vkaf283.2472
Abstract
Abstract Description
The annual healthcare cost of arthritis in the US is approximately $300 billion, and we are unable to prevent or reverse cartilage damage. This damage is likely caused by inflammation secondary to immune cell activity in the joint space. Our objective is to understand the underlying mechanisms of infectious and inflammatory arthritis. To study the inflammatory origin of human arthritis, we created an ex vivo model that recapitulates changes to the synovium seen in vivo. This system allows us to explore the changes to the unique immune cells lining the joint space: resident synovial macrophages (RSMs). RSMs are bound together by tight junctions into an intimal lining that protects the joint space from inflammation. Here, we demonstrate that infectious (Lipopolysaccharide, LPS) and inflammatory (Monocyte Chemoattractant Protein-1, MCP-1) stimuli disrupt synovial architecture. Specifically, LPS thinned the intimal lining while MCP-1 thickened it, and this may correspond with loss or gain of cells respectively. Both treatments decreased intimal border integrity. We conclude that observed changes in the synovial architecture are indicative of disruption to the protective joint lining. This would potentially allow circulating cells to gain entry to synovial fluid, thereby causing inflammatory damage to cartilage, bone, and synovium.
Topic Categories
Immune Mechanisms of Human Disease (HUM)
Details
- Title: Subtitle
- The effect of infectious and inflammatory stimuli on the synovium and resident synovial macrophages in a novel human explant model 9007
- Creators
- Katherine Lea Butler - University of IowaChristy Petersen - The Ohio State UniversityJacob Elkins - University of IowaRachel Johnson - University of IowaKaren I. Cyndari - University of Iowa
- Resource Type
- Abstract
- Publication Details
- The Journal of immunology (1950), Vol.214(Supplement_1), vkaf2832472
- DOI
- 10.1093/jimmun/vkaf283.2472
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Publisher
- Oxford University Press
- Alternative title
- IMMUNOLOGY2025™ Abstracts
- Language
- English
- Date published
- 11/01/2025
- Academic Unit
- Epidemiology; Emergency Medicine; Orthopedics and Rehabilitation; Internal Medicine
- Record Identifier
- 9985034934702771
Metrics
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