Abstract
Toxicity assessment of spark-generated, airborne copper oxide nanoparticles in lung epithelial cells
The European respiratory journal, Vol.44(Suppl 58), p.P1489
09/2014
DOI: 10.1183/13993003/erj.44.Suppl_58.P1489
Abstract
Abstract only Background: Copper oxide nanoparticles (CuONPs) are of great interest in nanotoxicology due to widespread use and significant toxicity.The aim of this study was to evaluate and compare the toxicity of CuONPs in human primary cells and in a commonly-employed cancer cell line. We investigated the consequences of CuONPs exposure using an in vitro air-liquid interface system. Methods: Human Bronchial Epithelial Cells (HBEC) and A549 cells were exposed to clean air or spark-generated CuONPs for 2 or 4 h. Cellular doses of Cu were assessed by inductively coupled plasma mass spectrometry. Outcome measures included cell viability, cytotoxicity (lactate dehydrogenase [LDH]), oxidative stress (reactive oxygen species [ROS]) and inflammation (interleukin [IL]-8). Results: The number median diameterand concentration of CuONPswere 9.2 nm and 3.5×10 7 particles/cm 3 . Compared with control, CuONPs significantly reduced cell viability, increased LDH release and elevated levels of ROS and IL-8 in a dose dependent manner. 4 hr exposure to CuONPs resulted in 63% loss of viability, 1.9-fold increase of ROS and 290% LDH release over controls in A549 cells; whereas HBEC had 15% viability loss, 2.7-fold ROS increase and 165% LDH release (all p<0.001 compared with A549); IL-8 was comparable in two cell types. N -acetylcysteine reduced the cell viability loss from 63% to 16% (p<0.001) and decreased ROS level from 190% to 132% (p<0.001). Conclusion: Spark-generated CuONPs exposure induced cytotoxicity, oxidative stress and inflammation in both cell types in a dose dependent manner. A549 cells were more susceptible to CuONPs effects than primary HBEC. Antioxidant treatment reduced CuONPs-induced cytotoxicity.
Details
- Title: Subtitle
- Toxicity assessment of spark-generated, airborne copper oxide nanoparticles in lung epithelial cells
- Creators
- Peter Thorne - University of IowaXuefang Jing - University of IowaJae Hong Park - University of IowaAndrea Adamcakova-Dodd - University of IowaThomas Peters - University of IowaSarah Perry - University of Iowa
- Resource Type
- Abstract
- Publication Details
- The European respiratory journal, Vol.44(Suppl 58), p.P1489
- DOI
- 10.1183/13993003/erj.44.Suppl_58.P1489
- ISSN
- 0903-1936
- eISSN
- 1399-3003
- Language
- English
- Date published
- 09/2014
- Academic Unit
- Occupational and Environmental Health
- Record Identifier
- 9985241078802771
Metrics
1 Record Views