Abstract
VCAN accumulation and proteolysis as predictors of T lymphocyte-excluded and permissive tumor microenvironments
Journal of clinical oncology, Vol.38(15_suppl), pp.3127-3127
05/20/2020
DOI: 10.1200/JCO.2020.38.15_suppl.3127
Abstract
Abstract only
3127
Background: Immune checkpoint inhibitors (ICIs) represent a major advance for treating solid tumors. However, only a minority of patients (pts) benefit from these therapies and markers that predict response have been elusive. Versican (VCAN) is an immunosuppressive proteoglycan in the tumor microenvironment (TME), which releases an immunostimulatory N-terminal fragment versikine (Vkine) when cleaved by ADAMTS proteases. We have demonstrated in colorectal cancers (CRC) that a low VCAN/high Vkine (VCAN proteolytic predominant [VPP]) phenotype correlates with increased tumor-infiltrating CD8
+
T lymphocytes (TILs). Here we examine the accumulation of VCAN as a marker of immune exclusion and its proteolysis as a marker of an immune-permissive TME. Methods: Immunohistochemistry for VCAN, Vkine and CD8+ was performed on samples from 1662 pts across breast (BC), CRC, endometrial cancer, pancreatic adenocarcinoma (PDAC), esophageal cancers and neuroendocrine tumors (NETs), across stages of disease (I-IV) and with diverse prior treatments. Stromal intensities of VCAN and Vkine staining quantified in collaboration with blinded surgical pathologists using a 0-3+ scale. 0/1+ were considered “low” for both VCAN and Vkine, whereas 2/3+ were considered “high”. The number of CD8
+
TILs were counted using 400x magnification, the equivalent of a high power field (hpf). Results: Across the entire cohort VCAN phenotypes were diverse (VCAN high/Vkine low, 21%; VCAN high/Vkine high, 23%; VCAN low/Vkine low, 29%; VCAN low/Vkine high (VPP), 27%). Consistent with VCAN accumulation as a marker of T cell exclusion, VCAN low cancers had increased TILs compared to VCAN high (4.8 vs 18.3 TILs/hpf, p < 0.001). Low VCAN was identified in 85% esophageal, 79% NET (including small cell lung cancer [SCLC]) 72% endometrial, 47% MSI-H CRCs, 28% triple-negative BC and only 22% MSS CRC, 18% PDAC, 17% ER+ BCs. The VPP subgroup had the highest TILs per hpf across tumors. VPP was identified in 47% of esophageal, 45% endometrial, 41% NETs (including SCLC), 24% MSI-H CRCs, and only 9% MSS CRC, 7% ER+ BCs, 3% triple-negative BCs, and 0% of PDAC (n = 131 PDAC pts). Conclusions: VCAN accumulation correlates with T lymphocyte exclusion, while VCAN proteolysis predicts an immune permissive phenotype. VCAN accumulation and proteolysis are now incorporated into ICI clinical trials as a potential marker of response. Future studies will clarify the role of these biomarkers in predicting benefits of immuno-oncology treatment strategies.
Details
- Title: Subtitle
- VCAN accumulation and proteolysis as predictors of T lymphocyte-excluded and permissive tumor microenvironments
- Creators
- Philip Emmerich - University of Wisconsin Carbone Cancer CenterKristina A. Matkowskyj - University of Wisconsin Carbone Cancer CenterStephanie McGregor - University of Wisconsin Carbone Cancer CenterSean Kraus - University of Wisconsin Carbone Cancer CenterKristen Bischel - University of Wisconsin Carbone Cancer CenterTonela Qyli - University of Wisconsin Carbone Cancer CenterDarya Buehler - University of Wisconsin Carbone Cancer CenterCheri Pasch - University of Wisconsin Carbone Cancer CenterChristopher Babiarz - University of Wisconsin Carbone Cancer CenterMitchell Depke - University of Wisconsin Carbone Cancer CenterLinda Clipson - University of Wisconsin Carbone Cancer CenterKari Braun Wisinski - University of Wisconsin Carbone Cancer CenterMark E. Burkard - University of Wisconsin Carbone Cancer CenterAndrew Baschnagel - University of Wisconsin Carbone Cancer CenterNataliya Volodymyrivna Uboha - University of Wisconsin Carbone Cancer CenterFotis Asimakopoulos - University of California, San DiegoDustin A. Deming - University of Wisconsin Carbone Cancer Center
- Resource Type
- Abstract
- Publication Details
- Journal of clinical oncology, Vol.38(15_suppl), pp.3127-3127
- DOI
- 10.1200/JCO.2020.38.15_suppl.3127
- ISSN
- 0732-183X
- eISSN
- 1527-7755
- Number of pages
- 1
- Language
- English
- Date published
- 05/20/2020
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984701249502771
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