Abstract
Warm Ischemia Time During Pediatric Kidney Transplant Does Not Correlate With Urine AKI Biomarkers: TH-PO098
Journal of the American Society of Nephrology, Vol.33(11S), pp.77-77
11/2022
DOI: 10.1681/ASN.20223311S177a
Abstract
Background:
Ischemia-reperfusion injury (IRI) is a mechanism of acute kidney injury (AKI). Like cardiopulmonary bypass, kidney transplantation is a form of controlled IRI resulting in acute tubular necrosis and possibly delayed graft function (DGF). Therefore, we sought to investigate kidney transplantation in pediatric patients as a clinical model of AKI, focusing on warm ischemia time (WIT) and novel AKI urine biomarkers.
Methods:
Prospective study of patients aged 3 months to 26 years who received a kidney transplant from 7/2020 and 11/2021. Urine was collected daily for 7 days post-transplant. Biomarkers tested were neutrophil gelatinase-associated lipocalin (NGAL), kidney injury molecule 1 (KIM-1), interleukin 18 (IL-18), and hepcidin. DGF was defined as receiving kidney replacement therapy within 7 days post-transplant. Wilcoxon rank sum test was used to compare cohorts. Linear regression was used to compare biomarker concentrations to WIT on post-operative days 0-2. The optimal cutoff for NGAL to predict lack of DGF was determined using negative predictive value (NPV) and a Youden's index.
Results:
4/30 patients developed DGF with a longer WIT of 55 minutes (IQR 52-57) vs. 38 minutes (IQR 32-45). Thus, we compared biomarker concentrations in patients with WIT <45 vs. ≥45 minutes. NGAL, KIM-1 and Hepcidin did not differ between cohorts. IL-18 was higher on day 1 in the ≥45-minute WIT cohort. A positive linear relationship was noted between WIT and NGAL on day 2 and a negative relationship with hepcidin on day 0. The optimal Youden's index was 0.6 for an NGAL cutoff of 150 ng/mL on either day 0 or 1 post-transplant with a NPV of 95.7% (95% CI 80-99%).
Conclusions:
We found no consistent correlation between WIT and biomarkers suggesting that AKI may be more complex than simply IRI. NGAL <150 ng/mL on post-operative day 0 or 1 correlated to a high NPV for development of DGF.
Details
- Title: Subtitle
- Warm Ischemia Time During Pediatric Kidney Transplant Does Not Correlate With Urine AKI Biomarkers: TH-PO098
- Creators
- Kyle Merrill - Cincinnati Children's Hospital Medical CenterCassie L. Kirby - Cincinnati Children's Hospital Medical CenterLakhmir S. Chawla - VA San Diego Healthcare SystemDavid K. Hooper - Cincinnati Children's Hospital Medical CenterStuart Goldstein - Cincinnati Children's Hospital Medical Center
- Resource Type
- Abstract
- Publication Details
- Journal of the American Society of Nephrology, Vol.33(11S), pp.77-77
- DOI
- 10.1681/ASN.20223311S177a
- ISSN
- 1046-6673
- eISSN
- 1533-3450
- Language
- English
- Date published
- 11/2022
- Academic Unit
- Stead Family Department of Pediatrics; Nephrology, Dialysis and Transplantation
- Record Identifier
- 9984652255002771
Metrics
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