Zinc Oxide Nanparticles Induce Apoptosis and Necrosis in Hepatocellular Carcinoma HepG2 Cells

Satya Murthy Tadinada; Maria B Lai; Vinaykumar Idikuda; Krishnaveni Mukka; Ratan Mani Singh; Jean Pfau; Alok Bhushan; Solomon Leung; James C.K. Lai

doi:10.1096/fasebj.27.1_supplement.1106.5

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Zinc Oxide Nanparticles Induce Apoptosis and Necrosis in Hepatocellular Carcinoma HepG2 Cells

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Zinc Oxide Nanparticles Induce Apoptosis and Necrosis in Hepatocellular Carcinoma HepG2 Cells

Satya Murthy Tadinada, Maria B Lai, Vinaykumar Idikuda, Krishnaveni Mukka, Ratan Mani Singh, Jean Pfau, Alok Bhushan, Solomon Leung and James C.K. Lai

The FASEB journal, Vol.27(S1)

04/2013

DOI: 10.1096/fasebj.27.1_supplement.1106.5

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Abstract

Abstract only Targeted drug delivery systems with minimal potential adverse/side effects are gaining utility in diverse applications. Uses of nanomaterials to design such targeted drug delivery are escalating exponentially. Nevertheless, the biocompatibility of nanomaterials is inadequately addressed. Consequently, among our systematic investigation of putative cytotoxic effects of nanomaterials in mammalian cell types, this study aims to elucidate mechanisms underlying the putative cytotoxic effects of ZnO nanoparticles on hepatocellular carcinoma HepG2 cells. Our results demonstrate that these nanoparticles exerted dose‐related decreases in survival of HepG2 cells. Findings from flow cytometry analysis and lactate dehydrogenase release assays reveal that the mode of cell death underlying the effects of the nanoparticle was a combination of apoptotic and necrotic cell death, while the percentage of necrotic cells predominated at higher treatment concentrations. Thus, our findings may have pathophysiological implications in biocompatibility of ZnO nanoparticles.

Details

Title: Subtitle: Zinc Oxide Nanparticles Induce Apoptosis and Necrosis in Hepatocellular Carcinoma HepG2 Cells
Creators: Satya Murthy Tadinada - Idaho State University
Maria B Lai - Idaho State University
Vinaykumar Idikuda - Idaho State University
Krishnaveni Mukka - Idaho State University
Ratan Mani Singh - Idaho State University
Jean Pfau - Idaho State University
Alok Bhushan - Idaho State University
Solomon Leung - Idaho State University
James C.K. Lai - Idaho State University
Resource Type: Abstract
Publication Details: The FASEB journal, Vol.27(S1)
DOI: 10.1096/fasebj.27.1_supplement.1106.5
ISSN: 0892-6638
eISSN: 1530-6860
Language: English
Date published: 04/2013
Academic Unit: Internal Medicine
Record Identifier: 9984966788402771

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