Book chapter
20 - Neuropeptide Conjugation to Carrier Proteins
Methods in Neurosciences, pp.333-351
1993
DOI: 10.1016/B978-0-12-185275-7.50025-1
Abstract
Neuropeptides are synthesized, stored, and secreted by neurons of the central and peripheral nervous system. A few neuropeptide receptors have been cloned and functionally expressed in frog oocytes; however, in most cases, the mechanism of action is unknown. Neuropeptides conjugated to proteins or other carriers, such as liposomes, may be used for targeting radioisotopes or drugs to cells, specialized endothelia, and normal or neoplastic tissues expressing the corresponding binding sites for diagnostic or therapeutic purposes. Studies regarding the immunocytochemistry of neuropeptides require antibodies of high avidity and specificity that must be able to differentiate among closely related molecules and to recognize their corresponding neuropeptides in tissue sections after a variety of pretreatments. For co-localization of neuropeptides with amino acid transmitters, catecholamines, or other amino group-free molecules, special fixation protocols are necessary. Depending on the chemical nature of the neuropeptide under consideration, these pretreatments inevitably lead to its chemical modification. This chapter describes the selected procedures for the conjugation of neuropeptides to carriers.
Details
- Title: Subtitle
- 20 - Neuropeptide Conjugation to Carrier Proteins
- Creators
- Rüdiger W. VehHans-Werner HabbesKarl-Heinz MeyerJörg CzekallaIsabella M. GrumbachHoang T. Pham
- Resource Type
- Book chapter
- Publication Details
- Methods in Neurosciences, pp.333-351
- DOI
- 10.1016/B978-0-12-185275-7.50025-1
- ISSN
- 1043-9471
- Language
- English
- Date published
- 1993
- Academic Unit
- Internal Medicine; Cardiovascular Medicine; Radiation Oncology
- Record Identifier
- 9984359679702771
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