Book chapter
[24] Aryl sulfotransferases
Methods in Enzymology, pp.197-206
Elsevier Science & Technology
1981
DOI: 10.1016/S0076-6879(81)77026-5
PMID: 6948989
Abstract
Aryl Sulfotransferases are a group of enzymes that are active with a wide variety of phenolic compounds as substrates. The aryl sulfotransferases fall into two families. The first family consists of aryl sulfotransferases I and II. Second family comprises of aryl sulfotransferases III and IV, which also resemble each other in their specificity for phenols. This chapter presents a procedure for the preparation and purification of these two families of aryl sulfotransferases. In preparation, the assay takes advantage of the partition into an organic solvent of the ion pair formed between certain organic sulfate monoesters and methylene blue. 2-Naphthol and adenosine 3'-phosphate 5'-phosphosulfate serve as substrates. The purification of all four aryl sulfotransferases is conducted together until the stage of partial separation upon elution from diethylaminoethyl cellulose /cellulose. The procedure has been applied to about 50 rat livers (0.5 kg) obtained from well-fed, male Sprague–Dawley TM rats weighing about 200 g. Livers are maintained at -80 ° prior to use.
Details
- Title: Subtitle
- [24] Aryl sulfotransferases
- Creators
- Ronald D. SekuraMichael W. DuffelWilliam B. Jakoby
- Resource Type
- Book chapter
- Publication Details
- Methods in Enzymology, pp.197-206
- Publisher
- Elsevier Science & Technology
- DOI
- 10.1016/S0076-6879(81)77026-5
- PMID
- 6948989
- eISSN
- 1557-7988
- ISSN
- 0076-6879
- Language
- English
- Date published
- 1981
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984303285902771
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