Book chapter
[3] Preparation of tyrosinamide—oligosaccharides as iodinatable glycoconjugates
Methods in Enzymology, pp.43-55
Elsevier Science & Technology
1994
DOI: 10.1016/S0076-6879(94)47005-7
PMID: 7534865
Abstract
This chapter describes a protocol that is an approach to isolate large quantities of reducing N-linked oligosaccharides from glycoproteins utilizing a batch procedure. The oligosaccharides are recovered as a protein-free mixture, converted to oligosaccharide-glycosylamines, and then conjugated with tyrosine. The tyrosinamide oligosaccharides are resolved into single structures on preparative RP-HPLC. Each major oligosaccharide contains a β-glycosylamide linkage between GIcNAc and the carboxyl group of tyrosine with only minor amounts (<5%) of α isomer recovered. The approach is versatile enough to allow the derivatization of sialyloligosaccharides and is capable of resolving complicated oligosaccharide mixtures after removing Boc. This modification also exposes a primary amine that can be further derivatized if desired.
Details
- Title: Subtitle
- [3] Preparation of tyrosinamide—oligosaccharides as iodinatable glycoconjugates
- Creators
- Toshiaki TamuraManpreet S WadhwaMing H ChiuM.L Corradi Da SilvaTamara McBroomKevin G Rice
- Resource Type
- Book chapter
- Publication Details
- Methods in Enzymology, pp.43-55
- DOI
- 10.1016/S0076-6879(94)47005-7
- PMID
- 7534865
- NLM abbreviation
- Methods Enzymol
- eISSN
- 1557-7988
- ISSN
- 0076-6879
- Publisher
- Elsevier Science & Technology
- Language
- English
- Date published
- 1994
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984065324302771
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