Book chapter
[3] Use of PCR for isolation of neuropeptide receptor genes
Methods in Neurosciences, pp.29-44
1995
DOI: 10.1016/S1043-9471(06)80081-9
Abstract
This chapter discusses the use of polymerase chain reaction (PCR) for the isolation of neuropeptide receptor genes. A complementary DNA (cDNA) encoding the pituitary adenylate cyclase-activating polypeptide (PACAP) receptor has been synthesized, which is a member of the vasoactive intestinal peptide (VIP)/secretin/parathyroid hormone (PTH)/calcitonin receptor family. The cloning of the VIP, secretin, PTH, and calcitonin receptors suggests that these receptors represent a new family of receptors in the superfamily of seven transmembrane-spanning G-protein-coupled receptors. Sequence comparison of these receptors revealed considerable homologies in their transmembrane domains. It has been hypothesized that these conserved sequences are a general structural feature of the receptors in this family and that these sequences would be conserved in the PACAP receptor. Thus, these conserved sequences have been targeted to design PCR primers in an effort to amplify gene segments for the PACAP receptor and other novel receptors within this family. Based on sequence information obtained from sequencing cDNA clones obtained by PCR with degenerate primers, PCR has been employed to isolate a full-length cDNA encoding the PACAP receptor. The chapter describes detailed procedures of this novel PCR strategy for molecular cloning of new neurotransmitter receptor cDNAs.
Details
- Title: Subtitle
- [3] Use of PCR for isolation of neuropeptide receptor genes
- Creators
- Tapan K ChatterjeeGobinda SarkarRory A FisherMark E Bolander - Department of Orthopedics, Mayo Clinic, Rochester, Minnesota 55905, USA
- Resource Type
- Book chapter
- Publication Details
- Methods in Neurosciences, pp.29-44
- DOI
- 10.1016/S1043-9471(06)80081-9
- ISSN
- 1043-9471
- Language
- English
- Date published
- 1995
- Academic Unit
- Internal Medicine; Iowa Neuroscience Institute; Neuroscience and Pharmacology
- Record Identifier
- 9984070960102771
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