Book chapter
[43] Detection of endotoxin by enhancement with toxic shock syndrome toxin-1 (TSST-1)
Methods in Enzymology, pp.302-306
Elsevier Science & Technology
1988
DOI: 10.1016/S0076-6879(88)65046-4
PMID: 3231108
Abstract
This chapter describes the detection of endotoxin by enhancement with toxic shock syndrome toxin-1 (TSST-1). Toxic shock syndrome (TSS) is an illness characterized by an array of clinical symptoms including fever, scarlatiniform rash, desquamation, multisystem involvement, and hypotension or shock. The pathogenesis of TSS is attributed to an exotoxin, TSST-1, produced by Staphylococcus aureus. There are at least three mechanisms by which TSST-1 can enhance the biological effects of endotoxin. First, TSST-1 is immunosuppressive, thereby allowing increased growth of gram-negative opportunistic organisms. This results in greater than normal amounts of endotoxin entering the systemic circulation. Second, the interaction of TSST-1 with the reticuloendothelial system hinders the clearance of endotoxin from the circulation. Third, TSST-1 can directly enhance the cytotoxicity of endotoxin. The combination of these effects demonstrated in vivo and in vitro results in lethal endotoxin shock in experimental animals. Endotoxin-free TSST-1 is prepared from cultures of toxigenic strains of Staphylococcus aureus grown in dialyzable beef heart medium.
Details
- Title: Subtitle
- [43] Detection of endotoxin by enhancement with toxic shock syndrome toxin-1 (TSST-1)
- Creators
- G.A BohachP.M Schlievert
- Resource Type
- Book chapter
- Publication Details
- Methods in Enzymology, pp.302-306
- Publisher
- Elsevier Science & Technology
- DOI
- 10.1016/S0076-6879(88)65046-4
- PMID
- 3231108
- eISSN
- 1557-7988
- ISSN
- 0076-6879
- Language
- English
- Date published
- 1988
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984001115902771
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