Book chapter
[6] Preparation of toxic shock syndrome toxin-1
Methods in Enzymology, pp.37-43
Elsevier Science & Technology
1988
DOI: 10.1016/S0076-6879(88)65009-9
PMID: 3231114
Abstract
This chapter describes the preparation of toxic shock syndrome toxin-1(TSST-1). The chapter discusses two methods for the purification of TSST-I. These two procedures differ primarily in their means of isolation, isoelectric focusing versus ion-exchange chromatography followed by gel filtration, rather than in culture methods. The isolation of TSST-1 by isoelectric focusing yields a highly purified product, as assessed by highly sensitive silver-staining techniques. By successive electrofocusing steps, TSST-1 is resolved into two separate bands, designated TSST-la and TSST-lb. Toxic shock syndrome (TSS) is a multisystem illness characterized by acute onset of high fever, hypotension or dizziness, rash, desquamation of the skin upon recovery, and variable multisystem involvement. TSST-1 is cited by several investigators as a major toxin most likely responsible for the symptoms of TSS. All reagents and glassware used in the purification of TSST-1 are maintained pyrogen free to prevent endotoxin contamination. Pyrogen-free water is prepared by glass-distilling deionized water. Staphylococcus aureus strain MN8 is used primarily as the source of TSST-1, TSST-la, and TSST-lb.
Details
- Title: Subtitle
- [6] Preparation of toxic shock syndrome toxin-1
- Creators
- Debra A Blomster-HautamaaPatrick M Schlievert
- Resource Type
- Book chapter
- Publication Details
- Methods in Enzymology, pp.37-43
- Publisher
- Elsevier Science & Technology
- DOI
- 10.1016/S0076-6879(88)65009-9
- PMID
- 3231114
- eISSN
- 1557-7988
- ISSN
- 0076-6879
- Language
- English
- Date published
- 1988
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984001115402771
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