Book chapter
A Dual-Readout High-Throughput Screening Assay for Small Molecules Active Against Aspergillus Fumigatus
Antifungal Drug Resistance, pp.35-42
Methods in molecular biology (Clifton, N.J.), 2658, Humana
2023
DOI: 10.1007/978-1-0716-3155-3_3
PMID: 37024693
Abstract
Human fungal infections caused by molds have been on the rise in recent years. These infections have high mortality rates compared to other fungal infections, and yet treatment options are limited due to resistance to clinical antifungals and lack of broad-spectrum activity against molds. Technical challenges associated with molds have limited large-throughput screening efforts for anti-mold compounds: therefore, we adapted an assay for use with A. fumigatus to help fill the gap in robust screening platforms for these organisms. This assay measures the release of the cytosolic enzyme adenylate kinase (AK) as a measure of fungal cell lysis and can also detect inhibition of germination as a reduction in the secretion of AK during vegetative growth. The ability to detect both lysis and inhibition of germination facilitates the identification of a wide range of compounds with different mechanisms of action, creating a strong screening platform for the identification of novel, anti-mold compounds.
Details
- Title: Subtitle
- A Dual-Readout High-Throughput Screening Assay for Small Molecules Active Against Aspergillus Fumigatus
- Creators
- Sarah R Beattie - Departments of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, IA, USA. sarah-r-beattie@uiowa.eduDamian J Krysan - University of Iowa
- Resource Type
- Book chapter
- Publication Details
- Antifungal Drug Resistance, pp.35-42
- Publisher
- Humana; New York, NY
- Series
- Methods in molecular biology (Clifton, N.J.); 2658
- DOI
- 10.1007/978-1-0716-3155-3_3
- PMID
- 37024693
- ISSN
- 1064-3745
- eISSN
- 1940-6029
- Language
- English
- Date published
- 2023
- Academic Unit
- Molecular Physiology and Biophysics; Microbiology and Immunology; Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
- Record Identifier
- 9984385054202771
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