Book chapter
A Toolkit of Protein-Fragment Complementation Assays for Studying and Dissecting Large-Scale and Dynamic Protein–Protein Interactions in Living Cells
Guide to Yeast Genetics: Functional Genomics, Proteomics, and Other Systems Analysis, pp.335-368
Methods in Enzymology, v. 470, Elsevier, Second edition
2010
DOI: 10.1016/S0076-6879(10)70014-8
PMID: 20946817
Abstract
Protein-fragment complementation assays (PCAs) are a family of assays for detecting protein-protein interactions (PPIs) that have been developed to provide simple and direct ways to study PPIs in any living cell, multicellular organism or in vitro. PCAs can be used to detect PPI between proteins of any molecular weight and expressed at their endogenous levels. Proteins are expressed in their appropriate cellular compartments and can undergo any posttranslational modification or degradation that, barring effects of the PCA fragment fusion, they would normally undergo. Applications of PCAs in yeast have been limited until recently, simply because appropriate expression plasmids or cassettes had not been developed. However, we have now developed and reported on several PCAs in Saccharomyces cerevisiae that cover the gamut of applications one could envision for studying any aspect of PPIs. Here, we present detailed protocols for large-scale analysis of PPIs with the survival selection dihydrofolate reductase (DHFR) reporter PCA and a new PCA based on a yeast cytosine deaminase reporter that allows for both survival and death selection. This PCA should prove a powerful way to dissect PPIs. We then present a method to study spatial localization and dynamics of PPIs based on fluorescent protein reporter PCAs and finally, two luciferase reporter PCAs that have proved useful for studies of dynamics of PPIs.
Details
- Title: Subtitle
- A Toolkit of Protein-Fragment Complementation Assays for Studying and Dissecting Large-Scale and Dynamic Protein–Protein Interactions in Living Cells
- Creators
- Stephen W. Michnick - Université de MontréalPo Hien Ear - Université de MontréalChristian Landry - Université LavalMohan K. Malleshaiah - Université de MontréalVincent Messier - Université de Montréal
- Contributors
- J Weissman (Editor)C Guthrie (Editor)G R Fink (Editor)
- Resource Type
- Book chapter
- Publication Details
- Guide to Yeast Genetics: Functional Genomics, Proteomics, and Other Systems Analysis, pp.335-368
- Edition
- Second edition
- Series
- Methods in Enzymology; v. 470
- DOI
- 10.1016/S0076-6879(10)70014-8
- PMID
- 20946817
- NLM abbreviation
- Methods Enzymol
- eISSN
- 1557-7988
- ISSN
- 0076-6879
- Publisher
- Elsevier; Amsterdam
- Number of pages
- 34
- Language
- English
- Date published
- 2010
- Academic Unit
- Surgery
- Record Identifier
- 9984321867202771
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