Book chapter
Amino-terminal processing of Dictyostelium discoideum actin
Dictyostelium discoideum: Molecular Approaches to Cell Biology, Vol.28, pp.231-243
Methods in cell biology, 28
1987
DOI: 10.1016/s0091-679x(08)61648-9
PMID: 3600408
Abstract
This chapter describes two assays for studying the removal of the Acamino acid from the amino terminus of preactin, procedures for making the actin precursor, preparation of reagents necessary for the assay, and a procedure for partial purification of the processing enzyme from rat liver. Various approaches are presented for further investigation of this processing system. The method for producing [35S]methionine-labeled Dictyostelium actin precursor by translating actin messenger RNA (mRNA) in a rabbit reticulocyte lysate is discussed. The methods described enables to study the nature of the unusual posttranslational modifications occurring at the NH2 terminus of virtually all actins. It is likely this modification is important for actin function, as it essentially has been conserved throughout evolution. Understanding how this processing occurs may be extremely important in elucidating the manner in which actins combine with actin-binding proteins such as myosin and various actin accessory proteins, such as depactin, which have been shown to bind to actin at or near its amino terminus.
Details
- Title: Subtitle
- Amino-terminal processing of Dictyostelium discoideum actin
- Creators
- P A RubensteinK L RedmanL R SolomonD J Martin
- Resource Type
- Book chapter
- Publication Details
- Dictyostelium discoideum: Molecular Approaches to Cell Biology, Vol.28, pp.231-243
- Publisher
- United States
- Series
- Methods in cell biology; 28
- DOI
- 10.1016/s0091-679x(08)61648-9
- PMID
- 3600408
- ISSN
- 0091-679X
- Grant note
- GM-33689 / NIGMS NIH HHS
- Language
- English
- Date published
- 1987
- Academic Unit
- Stead Family Department of Pediatrics; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984025278502771
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