Analysis of DNA Strand Break Induction by PCB Metabolites in HL-60 Cells

Anandi Srinivasan; Parvaneh Espandiari; Matthias Festag; Pablo Steinberg; Larry W. Robertson; G. Ludewig

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Book chapter

Analysis of DNA Strand Break Induction by PCB Metabolites in HL-60 Cells

Anandi Srinivasan, Parvaneh Espandiari, Matthias Festag, Pablo Steinberg, Larry W. Robertson and G. Ludewig

PCBs: Recent Advances in Environmental Toxicology and Health Effects, pp.395-398

The University Press of Kentucky

02/05/2015

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Abstract

Commercial mixtures of polychlorinated biphenyls (PCBs) are complete carcinogens in rodents, and several of these mixtures and individual PCB congeners are efficacious in the promotion of hepatocarcinogensis. Issues of PCBs’ involvement in initiation of cancer are less clear. PCBs are metabolized to dihydroxy and quinone metabolites by liver microsomes. The oxidation of dihydroxy PCB metabolites is accompanied by the generation of reactive oxygen species (ROS) that leads to the formation of DNA strand breaks in cell-free systems. To study whether the genotoxic properties of PCB metabolites may also be detected in cells in culture, we employed the comet assay, a

Astronomy

Biochemistry

Biological Sciences

Cell Biology

Comets

Cytology

DNA

Genetics

Metabolism

Metabolites

Molecular Probes

Nucleic Acids

Physical Sciences

Stem Cells

Acids

Astronomical objects

Biology

Cells

Cellular metabolism

Chemical compounds

Chemicals

Chemistry

Cultured cells

Cyclic compounds

DNA adducts

DNA damage

Free radicals

Functional groups

Genetic mutation

Genetic research

Genetic variation

Hematopoietic stem cells

Nucleic acid probes

Population genetics

Quinones

Reactive oxygen species

Details

Title: Subtitle: Analysis of DNA Strand Break Induction by PCB Metabolites in HL-60 Cells
Creators: Anandi Srinivasan
Parvaneh Espandiari
Matthias Festag
Pablo Steinberg
Larry W. Robertson
G. Ludewig
Resource Type: Book chapter
Publication Details: PCBs: Recent Advances in Environmental Toxicology and Health Effects, pp.395-398
Publisher: The University Press of Kentucky; Lexington
Language: English
Date published: 02/05/2015
Academic Unit: Occupational and Environmental Health
Record Identifier: 9984214666902771

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