Book chapter
Analysis of Drosophila chromatin structure in vivo
Methods in Enzymology, pp.462-496
Elsevier Science & Technology
1999
DOI: 10.1016/S0076-6879(99)04028-8
PMID: 10372377
Abstract
This chapter presents the analysis of Drosophila chromatin structure in vivo. Gene activation in vivo is a complex process. The chapter discusses in detail approaches for mapping chromatin structure, both at the level of nucleosome arrays and at the level of base-pair resolution, using cells or nuclei isolated from Drosophila at different stages of the life cycle. An in vivo analysis of chromatin structure and its functional role in regulating the expression of a given gene requires the exploitation of genetic tools whereby the effects of sequence alterations within the putative regulatory region and of mutations in the proposed trans-acting regulatory proteins can be examined. The extensive genetic information available and the ability to return an altered gene to the genome by P-element transformation make Drosophila an excellent system for these types of functional studies. The chapter describes the process of identifying major embryonic chromatin structural features at specific loci.
Details
- Title: Subtitle
- Analysis of Drosophila chromatin structure in vivo
- Creators
- Iain L. Cartwright - University of Cincinnati Medical CenterDiane E. CrydermanDavid S. Gilmour - Biochemistry & Molecular BiologyLori A. PileLori L. WallrathJanet A. WeberSarah C.R. Elgin
- Resource Type
- Book chapter
- Publication Details
- Methods in Enzymology, pp.462-496
- Publisher
- Elsevier Science & Technology
- DOI
- 10.1016/S0076-6879(99)04028-8
- PMID
- 10372377
- eISSN
- 1557-7988
- ISSN
- 0076-6879
- Language
- English
- Date published
- 1999
- Academic Unit
- Biochemistry and Molecular Biology; University College Courses
- Record Identifier
- 9984288732802771
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