Book chapter
Calcium imaging in cell-cell signaling
Developmental Biology Protocols Volume 1, Vol.135, pp.253-261
Methods in molecular biology (Clifton, N.J.), 135, Humana Press
2000
DOI: 10.1385/1-59259-685-1:253
PMID: 10791322
Abstract
The development of fluorescent calcium (Ca2+) indicators has been a very powerful tool for looking at the role of this second messenger in signal transduction. The ability to load these indicators into cells in a nondisruptive manner allow for the visualization of intracellular-free Ca2+ ion ([Ca2+]i) in a living developing embryo (reviewed in refs. 1 and 2). The vertebrate zebrafish (Danio rerio) is ideally suited for fluorescent studies by virtue of its transparent embryos. The zebrafish has two phases of [Ca2+]i release in the first few hours of development. The first is a phase of dramatic long-lived [Ca2+]i elevations associated with the forming cleavage furrows during the first few cell divisions (3). After the 16–32-cell stage, a subset of cells displays rapid aperiodic [Ca2+]i increases, localized to the enveloping region (4), and persist until the 1000–2000-cell stage (4,5) (see Fig. 1).
Details
- Title: Subtitle
- Calcium imaging in cell-cell signaling
- Creators
- Diane C Slusarski - Department of Biological Sciences, University of Iowa, Iowa City, USAVictor G Corces
- Resource Type
- Book chapter
- Publication Details
- Developmental Biology Protocols Volume 1, Vol.135, pp.253-261
- Publisher
- Humana Press; United States
- Series
- Methods in molecular biology (Clifton, N.J.); 135
- DOI
- 10.1385/1-59259-685-1:253
- PMID
- 10791322
- ISSN
- 1064-3745
- eISSN
- 1940-6029
- Grant note
- GM35463 / NIGMS NIH HHS GM17484 / NIGMS NIH HHS
- Language
- English
- Date published
- 2000
- Academic Unit
- Biology
- Record Identifier
- 9983991982502771
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