Book chapter
Chapter 16 Preparative purification and utilization of tyrosinamide N-Glycan libraries
Journal of Chromatography Library, pp.535-570
2002
DOI: 10.1016/S0301-4770(02)80041-0
Abstract
This chapter describes the preparative purification and utilization of tyrosinamide N-glycan libraries. Many analytical techniques have been developed to determine the identity and relative quantity of N-glycans on 1 mg or less of a glycoprotein. In contrast to analytical separations of N-glycans, a completely different strategy is required to purify μ mol quantities of individual N-glycans from the gram quantities of a glycoprotein. Semi-preparative reversed-phase high-performance liquid chromatography (RP-HPLC) affords a sufficient capacity for isolating hundred mg quantities of natural products. However, N-glycans are hydrophilic and therefore do not interact appreciably with reversed-phase resins. The presence of a Boc-tyrosinamide group on N-glycans helps alter separation selectivity and detect by absorbance or fluorescence. These chromatographic attributes have facilitated the preparative resolution of N-glycans from glycoproteins apart from allowing the monitoring of the enzymatic transformation of isolated tyrosinamide N-glycans into rare or novel structures.
Details
- Title: Subtitle
- Chapter 16 Preparative purification and utilization of tyrosinamide N-Glycan libraries
- Creators
- Kevin G. RiceV. Hayden ThomasWendy T. Collard - University of Michigan
- Resource Type
- Book chapter
- Publication Details
- Journal of Chromatography Library, pp.535-570
- DOI
- 10.1016/S0301-4770(02)80041-0
- ISSN
- 0301-4770
- Language
- English
- Date published
- 2002
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984365887502771
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