Chapter 21 - Organotypic Hippocampal Slice Cultures as a Model of Posttraumatic Epileptogenesis

Yevgeny Berdichevsky; Joseph Glykys; Volodya Dzhala; Kyle Lillis; Kevin J Staley

doi:10.1016/B978-0-12-804066-9.00021-3

Back

Book chapter

Chapter 21 - Organotypic Hippocampal Slice Cultures as a Model of Posttraumatic Epileptogenesis

Yevgeny Berdichevsky, Joseph Glykys, Volodya Dzhala, Kyle Lillis and Kevin J Staley

Models of Seizures and Epilepsy, pp.301-311

Elsevier Inc, Second Edition

2017

DOI: 10.1016/B978-0-12-804066-9.00021-3

View Online

Abstract

Organotypic cultures are prepared by dissecting slices from the brain region of interest, and then maintaining the slices in appropriate culture conditions for time period ranging from 24 h to several weeks. These cultures have been prepared from a variety of brain regions and maintain general morphology of the originating brain region during the culture period. Spontaneous ictal and interictal epileptiform activity occurs in close to 100% of organotypic cultures from hippocampus and cortex. Therefore, these cultures are increasingly used as an in vitro model of epileptogenesis. Some of the advantages of this model include compressed time scale of epilepsy development and relatively easy experimental access. Organotypic cultures have been used by investigators to investigate mechanisms of epileptogenesis via calcium and chloride imaging, and to rapidly screen potential antiepileptic drugs.

Anticonvulsants

slice cultures

hippocampal

organotypic culture model

posttraumatic epileptogenesis

chloride imaging

drug screen

calcium imaging

Details

Title: Subtitle: Chapter 21 - Organotypic Hippocampal Slice Cultures as a Model of Posttraumatic Epileptogenesis
Creators: Yevgeny Berdichevsky - Lehigh University, Bethlehem, PA, United States
Joseph Glykys - Massachusetts General Hospital and Harvard Medical School, Boston, MA, United States
Volodya Dzhala - Massachusetts General Hospital and Harvard Medical School, Boston, MA, United States
Kyle Lillis - Massachusetts General Hospital and Harvard Medical School, Boston, MA, United States
Kevin J Staley - Massachusetts General Hospital and Harvard Medical School, Boston, MA, United States
Resource Type: Book chapter
Publication Details: Models of Seizures and Epilepsy, pp.301-311
Edition: Second Edition
DOI: 10.1016/B978-0-12-804066-9.00021-3
Publisher: Elsevier Inc
Language: English
Date published: 2017
Academic Unit: Neurology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Neurology (Pediatrics)
Record Identifier: 9984065774902771

Metrics

80 Record Views

1 Times Cited - Web of Science