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Chapter 43 - Intracellular pH homeostasis
Book chapter

Chapter 43 - Intracellular pH homeostasis

Ian M. Thornell, Marie Michenkova, Sara Taki, Mark O. Bevensee and Walter F. Boron
Seldin and Giebisch's The Kidney, pp.1561-1668
Academic Press, Sixth Edition
2026
DOI: 10.1016/B978-0-12-815389-5.00077-0

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Abstract

We shall first consider the methodologies available for making pHi measurements and then examine the factors that contribute to changes in pHi: the thermodynamic forces acting on hydrogen ions (H+) and other charged acids/bases; the permeation of the cell membrane by electrically neutral acids and bases; the buffering power of the intracellular fluid (ICF); and transporters that regulate pHi by moving H+, bicarbonate ions (HCO3–) and/or other weak acid/bases across the plasma membrane. Because space does not permit us to summarize the vast array of acid-base transporters, we will focus on those that play a major role in pHi homeostasis. Emerging from this discussion will be a model of pHi regulation. Finally, in light of this model, we will consider several factors that fundamentally alter pHi homeostasis: alterations in pHo; temperature changes; metabolic inhibitors and hypoxia; cell shrinkage; hormones, growth factors, and oncogenes, including cancer.
acid extrusion acid loading acid-base transport acid-base transporters buffering power measuring pHi pH physiology pHi regulation

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