Book chapter
Chapter 9.11 Contractile and Cytoskeletal Proteins
Journal of Chromatography Library, pp.172-176
1983
DOI: 10.1016/S0301-4770(08)61317-2
Abstract
This chapter describes applications of polyacrylamide gel electrofocusing and electrophoresis in cytoskeletal and contractile protein research, and selected examples of different uses of these techniques. Contractile and cytoskeletal proteins are present at high levels within the cell, and often they are not associated with an easily assayable enzymatic activity. By using polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS-PAGE), one can detect simultaneously actin, α-actinin, myosin heavy and light chains, tropomyosin, and troponin in skeletal and smooth muscle myofibril preparations. SDS–polyacrylamide gel electrophoresis and isoelectric focusing have proved extremely valuable in assaying the presence of families of contractile and cytoskeletal proteins, in determining the complexity of these multi component families, in studying the developmental regulation of the expression of these proteins, and in assessing their degree of post-translational modification.
Details
- Title: Subtitle
- Chapter 9.11 Contractile and Cytoskeletal Proteins
- Creators
- Peter Rubenstein
- Resource Type
- Book chapter
- Publication Details
- Journal of Chromatography Library, pp.172-176
- DOI
- 10.1016/S0301-4770(08)61317-2
- ISSN
- 0301-4770
- Language
- English
- Date published
- 1983
- Academic Unit
- Stead Family Department of Pediatrics; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984288727002771
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