Book chapter
Characterization of Islet Leukocyte Populations in Human and Murine Islets by Flow Cytometry
Methods in molecular biology (Clifton, N.J.), pp.185-197
Methods in Molecular Biology, Humana Press Inc
01/01/2020
DOI: 10.1007/978-1-4939-9882-1_10
PMCID: PMC9255050
PMID: 31586328
Abstract
An increasing body of evidence indicates that a local islet immune response is not only limited to type 1 diabetes, but also is associated with islet dysfunction in type 2 diabetes. Recently, the presence of pancreatic CD68(+) macrophages within islet tissues was demonstrated by RT-PCR and immunohistochemical methods. However, the precise profile and activation status of intraislet leukocytes, which are present in both murine and human islets, are poorly defined. Here, we describe a detailed flow cytometry protocol designed to analyze both human and murine islets for intraislet leukocytes and leukocyte subsets. This approach permits the simultaneous identification of multiple intraislet leukocyte subsets, as well as their activation statuses. The use of flow cytometry-based approaches will advance the field of islet biology and help to identify unique changes in the immune cell composition that accompanies pathological islet inflammation and dysfunction in type 2 diabetes.
Details
- Title: Subtitle
- Characterization of Islet Leukocyte Populations in Human and Murine Islets by Flow Cytometry
- Creators
- Matthew J. Butcher - Eastern Virginia Medical SchoolMichelle B. Trevino - Eastern Virginia Medical SchoolYumi Imai - Eastern Virginia Medical SchoolElena V. Galkina - Eastern Virginia Medical School
- Contributors
- C J Stocker (Editor)
- Resource Type
- Book chapter
- Publication Details
- Methods in molecular biology (Clifton, N.J.), pp.185-197
- Series
- Methods in Molecular Biology
- DOI
- 10.1007/978-1-4939-9882-1_10
- PMID
- 31586328
- PMCID
- PMC9255050
- NLM abbreviation
- Methods Mol Biol
- eISSN
- 1940-6029
- ISSN
- 1064-3745
- Publisher
- Humana Press Inc; TOTOWA
- Number of pages
- 13
- Grant note
- IIDP pilot program BD Biosciences research grant Eastern Virginia Medical School pilot grant RO1-DK090490 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 01/01/2020
- Academic Unit
- Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984360039302771
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