Book chapter
Characterizing Candida glabrata Pdr1, a Hyperactive Transcription Factor Involved in Azole Resistance
Antifungal Drug Resistance, p.169
Methods in molecular biology (Clifton, N.J.), 2658, Humana
2023
DOI: 10.1007/978-1-0716-3155-3_11
PMID: 37024701
Abstract
This chapter illustrates how to prepare isogenic strains carrying gain-of-function forms of transcription factor Pdr1 in the human pathogen Candida glabrata. Simple steps are described that lead from a characterized plasmid-borne PDR1-GOF allele to its integration into the yeast genome in a markerless manner. Pdr1-GOF strains constructed by this approach are suitable for virulence studies in an animal host.
Details
- Title: Subtitle
- Characterizing Candida glabrata Pdr1, a Hyperactive Transcription Factor Involved in Azole Resistance
- Creators
- Lucia Simonicova - University of IowaW Scott Moye-Rowley - Department of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, Iowa City, IA, USA. scott-moye-rowley@uiowa.edu
- Resource Type
- Book chapter
- Publication Details
- Antifungal Drug Resistance, p.169
- Publisher
- Humana; New York, NY
- Series
- Methods in molecular biology (Clifton, N.J.); 2658
- DOI
- 10.1007/978-1-0716-3155-3_11
- PMID
- 37024701
- ISSN
- 1064-3745
- eISSN
- 1940-6029
- Language
- English
- Date published
- 2023
- Academic Unit
- Molecular Physiology and Biophysics
- Record Identifier
- 9984385048602771
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