Book chapter
Chromosomal Protein Antigens Formed in Experimental Hepatocarcinogenesis by Azo Dyes
Progress in Nucleic Acid Research and Molecular Biology, pp.253-256
Elsevier Science & Technology
1983
DOI: 10.1016/S0079-6603(08)60454-7
PMID: 6420841
Abstract
This chapter examines chromosomal proteins immunologically during hepatocarcinogenesis in rats maintained on a diet containing azo dye. In this study, chromatin from several azo-dye hepatomas was pooled, dehistonized, and used to prepare heterogeneous antisera in rabbits. These antisera were then employed to assess various chromatins for the presence of antigens specific for neoplasia during induced carcinogenesis. All assays were performed using immunodetection of antigens, separated electrophoretically and transferred to nitrocellulose. The gradual development of neoplasia was accompanied by a chronological appearance of antigens until the complement of immunoreactive antigens characteristic of primary hepatomas was achieved. Examination of the Coomassie-stained gels of these chromatins showed that normal liver chromatin contained many polypeptides of similar mobility, but present in varying quantities with respect to that observed for the primary hepatomas. Mainly quantitative variations in nonhistone proteins with hepatocarcinogenesis have also been reported. However, the use of antibodies and the immunotransfer indicate that neoplasia may induce more extensive changes that are detectable immunologically. Fetal liver, regenerating liver, and several transplantable tumors were also examined in the study presented in the chapter. Fetal or regenerating liver chromatin showed more antigenic homology than normal liver or kidney chromatins, which may indicate that some of these antigens are associated with rapid cellular proliferation. In general, transplantable tumors contain more antigens than normal tissues.
Details
- Title: Subtitle
- Chromosomal Protein Antigens Formed in Experimental Hepatocarcinogenesis by Azo Dyes
- Creators
- W.N SchmidtB.J GronertR.C BriggsD.L PageL.S Hnilica
- Resource Type
- Book chapter
- Publication Details
- Progress in Nucleic Acid Research and Molecular Biology, pp.253-256
- Publisher
- Elsevier Science & Technology
- DOI
- 10.1016/S0079-6603(08)60454-7
- PMID
- 6420841
- ISSN
- 0079-6603
- Language
- English
- Date published
- 1983
- Academic Unit
- Gastroenterology and Hepatology; Internal Medicine
- Record Identifier
- 9984094659602771
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