Book chapter
Co-Immunoprecipitation: Isolation of Protein Signaling Complexes from Native Tissues
Laboratory Methods in Cell Biology: Biochemistry and Cell Culture, pp.33-54
Methods in Cell Biology, 112, Elsevier Science & Technology
2012
DOI: 10.1016/B978-0-12-405914-6.00002-0
Abstract
Immunoprecipitation (IP) is defined as the isolation of an antigen using a specific antibody coupled to a sedimentable matrix. The theory behind co-immunoprecipitation (Co-IP) is similar but specifically refers to the utilization of IP for the purpose of identifying the binding partners of a specific protein of interest or the detection of complex formation between two or more protein components. Often, investigations into protein binding partners will begin in a heterologous expression system where interactions are detected between overexpressed and/or tagged proteins. While such experiments represent a valid means to begin probing for complex formation between two or more proteins of interest, such experiments are subject to artifacts, and it is often essential to confirm that an interaction observed in cell culture also occurs in native tissue. The purpose of this protocol is to describe the use of Co-IP for isolation of protein complexes from a native tissue lysate in order to detect specific protein-protein interactions occurring in vivo. Such interactions are important in numerous physiological processes and are targeted by current and emerging therapeutics.
Details
- Title: Subtitle
- Co-Immunoprecipitation: Isolation of Protein Signaling Complexes from Native Tissues
- Creators
- Adele StewartRory A Fisher - University of Iowa, Neuroscience and Pharmacology
- Resource Type
- Book chapter
- Publication Details
- Laboratory Methods in Cell Biology: Biochemistry and Cell Culture, pp.33-54
- Publisher
- Elsevier Science & Technology; San Diego
- Series
- Methods in Cell Biology; 112
- DOI
- 10.1016/B978-0-12-405914-6.00002-0
- ISSN
- 0091-679X
- Number of pages
- 505
- Language
- English
- Date published
- 2012
- Academic Unit
- Iowa Neuroscience Institute; Internal Medicine; Neuroscience and Pharmacology
- Record Identifier
- 9984217059602771
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