Book chapter
Cool Tools 3: Large‐Scale Genetic Interaction Screening in Candida albicans
Candida and Candidiasis, pp.497-500
ASM Press
12/07/2011
DOI: 10.1128/9781555817176.ch32
Abstract
Although the genetic study of Candida albicans is generally less convenient than that of Saccharomyces cerevisiae, recent advances in the molecular genetics of C. albicans have greatly facilitated the study of this important human fungal pathogen. This chapter discusses the development of a novel strategy for large‐scale synthetic genetic analysis in C. albicans based on complex haploin‐sufficiency (CHI). An actin null mutant (act1?) containing ACT1 on a plasmid was mated to the set of single‐gene‐deletion strains and then cured of the plasmid to give a set of complex heterozygotes (act1Δ/ACT1 yfgΔ/YFG). Many of the genes identified using this genetic approach demonstrated defects in the actin cytoskeleton, confirming the ability of CHI to identify interacting pairs of genes and genes with similar cellular functions. The development of a large‐scale synthetic genetic screen using CHI to understand the role of the RAM network in C. albicans morphological transition is described. As new mutagenesis techniques are developed and as large collections of mutants are created, it is likely that the creative application of these principles to genetic interaction analysis will lead to a deeper understanding of the complex regulatory networks that orchestrate C. albicans biology and pathogenesis.
Details
- Title: Subtitle
- Cool Tools 3: Large‐Scale Genetic Interaction Screening in Candida albicans
- Creators
- Yeissa Chabrier‐RosellóAnuj Kumar - University of MichiganDamian J Krysan - University of Rochester
- Contributors
- Richard A Calderone (Editor)Cornelius J Clancy (Editor)
- Resource Type
- Book chapter
- Publication Details
- Candida and Candidiasis, pp.497-500
- DOI
- 10.1128/9781555817176.ch32
- Publisher
- ASM Press; Washington, DC, USA
- Number of pages
- 4
- Language
- English
- Date published
- 12/07/2011
- Academic Unit
- Microbiology and Immunology; Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
- Record Identifier
- 9984297545402771
Metrics
11 Record Views