Book chapter
Covalent Interaction of Synthetic Benzoquinones and Hydroquinones of Polychlorinated Biphenyls with DNA
PCBs, p.403
The University Press of Kentucky
02/05/2015
Abstract
Commercial polychlorinated biphenyls (PCBs) are complete carcinogens in rodents; however, their initiating (DNA damaging) activity has not been conclusively demonstrated. In the present study, we reacted synthetic 2-phenyl-1,4-benzoquinones of 2-chloro- (2-CBQ), 3-chloro- (3-CBQ), 4-chloro- (4-CBQ), 3,4-dichloro- (3,4-CBQ) and 3,4,5-trichloro (3,4,5-CBQ) biphenylsin vitrowith calf thymus DNA at pH 5.0, 7.4 and 9.5 for 4 h at 37 °C. Analysis of 4-CBQ-derived DNA adducts by nuclease PI-version of ³²P-postlabeling showed a similar adduct pattern at all the pHs tested; the reactivity with DNA was in the following descending order: pH 5.0 > pH 9.5 >> pH 7.4. A similar observation
Details
- Title: Subtitle
- Covalent Interaction of Synthetic Benzoquinones and Hydroquinones of Polychlorinated Biphenyls with DNA
- Creators
- Jamal M. ArifHans-Joachim LehmlerLarry W. RobertsonRamesh C. Gupta
- Contributors
- Howard Glauert (Editor)Ramesh Gupta (Editor)
- Resource Type
- Book chapter
- Publication Details
- PCBs, p.403
- Publisher
- The University Press of Kentucky
- Language
- English
- Date published
- 02/05/2015
- Academic Unit
- Occupational and Environmental Health; Iowa Neuroscience Institute
- Record Identifier
- 9984001086802771
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