Book chapter
Culturing Immortalized Human Airway Epithelial Cells at an Air-Liquid Interface for Measles Virus Infection
Measles and Related Morbilliviruses, pp.141-152
Methods in molecular biology (Clifton, N.J.), v. 2808, Humana
2024
DOI: 10.1007/978-1-0716-3870-5_11
Abstract
Measles virus (MeV) infection of airway surface epithelial cells provides a site for final amplification before being released back into the environment via coughing and sneezing. Multiple cell lines have served as models of polarized epithelia for MeV infection, such as Caco2 cells (intestinal derived human epithelia) or MDCK cells (kidney derived canine epithelia). In this chapter, we describe the materials and air-liquid interface (ALI) culture conditions for maintaining four different cell lines derived from human airway epithelial cells: 16HBE14o-, Calu-3, H358, and NuLi-1. We provide methods for confirming transepithelial electrical resistance (TER) and preparing samples for microscopy as well as expected results from apical or basolateral MeV delivery. Polarized human airway derived cells serve as tissue culture models for investigating targeted questions about how MeV exits a human host. In addition, these methods are generalizable to studies of other respiratory viruses or the biology of ALI airway epithelial cells.
Details
- Title: Subtitle
- Culturing Immortalized Human Airway Epithelial Cells at an Air-Liquid Interface for Measles Virus Infection
- Creators
- Justin W Kaufman - University of IowaBrajesh K Singh - University of IowaNi Li - Scripps Research InstitutePatrick L Sinn - University of Iowa
- Resource Type
- Book chapter
- Publication Details
- Measles and Related Morbilliviruses, pp.141-152
- Publisher
- Humana; New York, NY
- Series
- Methods in molecular biology (Clifton, N.J.); v. 2808
- DOI
- 10.1007/978-1-0716-3870-5_11
- eISSN
- 1940-6029
- Language
- English
- Date published
- 2024
- Academic Unit
- Microbiology and Immunology; Stead Family Department of Pediatrics; Pulmonary Medicine
- Record Identifier
- 9984626997002771
Metrics
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