Book chapter
Cysteamine and cystamine
Methods in Enzymology, pp.149-154
Elsevier Science & Technology
1987
DOI: 10.1016/0076-6879(87)43027-9
PMID: 3657527
Abstract
This chapter discusses about cysteamine and cystamine. Cysteamine (2-aminoethanethiol) is formed in mammalian tissues by the enzymatic hydrolysis of pantetheine, and has the role in the biosynthesis of hypotaurine. Cysteamine has been used therapeutically to treat cystinosis and sickle cell anemia, and has been used as a protective agent against hepatotoxins and ionizing radiation. However, despite its clinical applications, only a few procedures have been described for the determination of cysteamine in body fluids of animals treated with this aminothiol, and none are readily adaptable for measuring both cysteamine and its disulfide, cystamine, in a single sample. This chapter describes two different methods for the determination of these aminothiois. The first is a rapid, specific enzymatic procedure that is readily adapted for the simultaneous estimation of both cysteamine and cystamine in plasma from treated animals. The second is a more sensitive, but also more laborious, colorimetric method for the estimation of endogenous (protein-bound + free cysteamine) in animal tissues.
Details
- Title: Subtitle
- Cysteamine and cystamine
- Creators
- Michael W. DuffelDaniel J. LoganDaniel M. Ziegler
- Resource Type
- Book chapter
- Publication Details
- Methods in Enzymology, pp.149-154
- Publisher
- Elsevier Science & Technology
- DOI
- 10.1016/0076-6879(87)43027-9
- PMID
- 3657527
- eISSN
- 1557-7988
- ISSN
- 0076-6879
- Language
- English
- Date published
- 1987
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984303163902771
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