Book chapter
Dendritic Cells in Chronic In Vivo Ethanol Exposure Models
Alcohol, pp.213-233
Methods in Molecular Biology, Humana Press
2008
DOI: 10.1007/978-1-59745-242-7_16
PMID: 18369922
Abstract
Dendritic cells (DCs) play a key role in the initiation of effective immune responses against infectious agents because they are unique in their ability to provide antigen-specific activation of naïve T cells. To do this, they must acquire antigen and migrate to spleen or lymph node to present the antigen to T cells in association with costimulatory molecules and cytokines. Murine models of chronic EtOH exposure have been developed for dissecting the mechanisms by which EtOH alters immune cell functions. This chapter details methods for assessing DC functions in such models. Methods are presented for 1) the identification and isolation of various DC subsets from spleen, epidermis, and lung, 2) measurement of LC migration out of epidermis and DC migration into peripheral and peribronchial lymph nodes, and 3) measurement of alloantigen presentation in vitro as well as transgenic T-cell activation in vitro and in vivo.
Details
- Title: Subtitle
- Dendritic Cells in Chronic In Vivo Ethanol Exposure Models
- Creators
- Kevin L Legge - Department of Pathology, University of Iowa Carver College of Medicine, Iowa City, USAAnnette J Schlueter - Department of Pathology, University of Iowa Carver College of Medicine, Iowa City, USA
- Resource Type
- Book chapter
- Publication Details
- Alcohol, pp.213-233
- Publisher
- Humana Press; Totowa, NJ
- Series
- Methods in Molecular Biology
- DOI
- 10.1007/978-1-59745-242-7_16
- PMID
- 18369922
- eISSN
- 1940-6029
- ISSN
- 1064-3745
- Language
- English
- Date published
- 2008
- Academic Unit
- Microbiology and Immunology; Pathology
- Record Identifier
- 9984047680902771
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