Book chapter
Electrophoretic Mobility Shift Assay in Aspergillus fumigatus
Aspergillus fumigatus, Vol.3028, pp.143-152
Methods in molecular biology (Clifton, N.J.), v. 3028, Springer
2026
DOI: 10.1007/978-1-0716-5222-0_10
PMID: 41968250
Abstract
One of the classic techniques to allow detection of direct DNA-binding by sequence-specific transcription factors is the electrophoretic mobility shift assay (EMSA). EMSA takes advantage of the reduced mobility of a protein:DNA complex during gel electrophoresis compared to the naked DNA alone. This rapid and straightforward method has been used to identify DNA target elements in organisms ranging from bacteria to humans. Here we describe its use with a focus on the AtrR transcription factor from the filamentous fungus Aspergillus fumigatus.
Details
- Title: Subtitle
- Electrophoretic Mobility Shift Assay in Aspergillus fumigatus
- Creators
- Vijendra Arya - University of IowaSanjoy Paul - University of IowaW Scott Moye-Rowley - University of Iowa
- Resource Type
- Book chapter
- Publication Details
- Aspergillus fumigatus, Vol.3028, pp.143-152
- Series
- Methods in molecular biology (Clifton, N.J.); v. 3028
- DOI
- 10.1007/978-1-0716-5222-0_10
- PMID
- 41968250
- eISBN
- 1071652222; 9781071652220
- ISSN
- 1064-3745
- eISSN
- 1940-6029
- Publisher
- Springer; Boston
- Language
- English
- Date published
- 2026
- Academic Unit
- Molecular Physiology and Biophysics
- Record Identifier
- 9985153394702771
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