Book chapter
Evaluation of Activation and Inflammatory Activity of Myeloid Cells During Pathogenic Human Coronavirus Infection
MERS Coronavirus, pp.195-204
Methods in molecular biology (Clifton, N.J.), 2099, Humana
2020
DOI: 10.1007/978-1-0716-0211-9_15
PMCID: PMC7123665
PMID: 31883097
Abstract
Innate immune cells play a vital role in mounting an effective host response to a variety of pathogen challenges. Myeloid cells such as neutrophils and monocyte-macrophages are major innate leukocytes that orchestrate protective immunity to viral lung infections. However, a dysregulated cytokine response can promote excessive infiltration and robust pro-inflammatory activity of neutrophils and monocyte-macrophages, leading to fatal disease. Following virus infection, the beneficial or deleterious role of infiltrating neutrophils and monocyte-macrophages is determined largely by their ability to secrete inflammatory cytokines and chemokines. A majority of studies use the total number of infiltrating cells and their activation status as measures to demonstrate their role during an infection. Consequently, the ability of neutrophils and Inflammatory Monocyte Macrophages (IMMs) to secrete inflammatory cytokines and chemokines, and its correlation with the disease severity, is not well defined. In this chapter, we report useful markers to identify lung infiltrating innate immune cells and define their activation status. We also describe a simple method to measure intracellular cytokine production to evaluate the inflammatory activity of neutrophils and IMMs in a mouse model of human coronavirus infection.
Details
- Title: Subtitle
- Evaluation of Activation and Inflammatory Activity of Myeloid Cells During Pathogenic Human Coronavirus Infection
- Creators
- Rudragouda Channappanavar - Institute for the Study of Host-Pathogen Systems, University of Tennessee Health Science Center, Memphis, TN, USA. rchanna1@uthsc.eduStanley Perlman - University of Iowa, Stead Family Department of Pediatrics
- Resource Type
- Book chapter
- Publication Details
- MERS Coronavirus, pp.195-204
- Publisher
- Humana; New York, NY
- Series
- Methods in molecular biology (Clifton, N.J.); 2099
- DOI
- 10.1007/978-1-0716-0211-9_15
- PMID
- 31883097
- PMCID
- PMC7123665
- ISSN
- 1064-3745
- eISSN
- 1940-6029
- Language
- English
- Date published
- 2020
- Academic Unit
- Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
- Record Identifier
- 9983782099102771
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