Book chapter
Evaluation of the Adaptive Immune Response to Respiratory Syncytial Virus
Human Respiratory Syncytial Virus, pp.231-243
Methods in Molecular Biology, Springer New York
07/28/2016
DOI: 10.1007/978-1-4939-3687-8_17
PMID: 27464699
Abstract
Evaluation of the adaptive immune response is critical to the advancement of our basic knowledge and understanding of respiratory syncytial virus (RSV). The cellular composition in the lung following RSV infection is often evaluated using flow cytometry. However, a limitation of this approach has been the inability to readily distinguish cells that are within the lung parenchyma from cells that remain in the pulmonary blood vessels. Herein, we detail a procedure to evaluate the adaptive immune response via flow cytometric analysis that incorporates an in vivo intravascular staining technique. This technique allows for discrimination of immune cells in the lung tissue from cells that remain in the pulmonary vasculature following perfusion. Therefore at any given time point following an RSV infection, the leukocytic populations in the lung parenchyma can be quantified and phenotypically assessed with high resolution. While we focus on the T lymphocyte response in the lung, this technique can be readily adapted to examine various leukocytic cell types in the lung following RSV infection.
Details
- Title: Subtitle
- Evaluation of the Adaptive Immune Response to Respiratory Syncytial Virus
- Creators
- Cory J Knudson - Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, USAKayla A Weiss - Department of Microbiology and Immunology, Albert Einstein College of Medicine, New York, USAMegan E Stoley - Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, USASteven M Varga - Department of Pathology, University of Iowa, Iowa City, USA
- Resource Type
- Book chapter
- Publication Details
- Human Respiratory Syncytial Virus, pp.231-243
- Publisher
- Springer New York; New York, NY
- Series
- Methods in Molecular Biology
- DOI
- 10.1007/978-1-4939-3687-8_17
- PMID
- 27464699
- eISSN
- 1940-6029
- ISSN
- 1064-3745
- Language
- English
- Date published
- 07/28/2016
- Academic Unit
- Graduate College Admin and Gen; Microbiology and Immunology; Pathology
- Record Identifier
- 9984083276102771
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