Experimental Protocol for MRI Mapping of the Blood Oxygenation-Sensitive Parameters T2 and T2 in the Kidney - Chapter 23

Andreas Pohlmann; Kaixuan Zhao; Sean B Fain; Pottumarthi V Prasad; Thoralf Niendorf

doi:10.1007/978-1-0716-0978-1_23

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Experimental Protocol for MRI Mapping of the Blood Oxygenation-Sensitive Parameters T2 and T2 in the Kidney - Chapter 23

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Experimental Protocol for MRI Mapping of the Blood Oxygenation-Sensitive Parameters T2 and T2 in the Kidney - Chapter 23

Andreas Pohlmann, Kaixuan Zhao, Sean B Fain, Pottumarthi V Prasad and Thoralf Niendorf

Preclinical MRI of the Kidney: Methods and Protocols

Methods in Molecular Biology , volume 2216, Springer US

2021

DOI: 10.1007/978-1-0716-0978-1_23

PMCID: PMC9703278

PMID: 33476013

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https://doi.org/10.1007/978-1-0716-0978-1_23View

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Abstract

Renal hypoxia is generally accepted as a key pathophysiologic event in acute kidney injury of various origins, and has also been suggested to play a role in the development of chronic kidney disease. Here we describe a step-by-step experimental protocol for indirect monitoring of renal blood oxygenation in rodents via the deoxyhemoglobin sensitive MR parameters T2* and T2—a contrast mechanism known as the blood oxygenation level dependent (BOLD) effect. Since an absolute quantification of renal oxygenation from T2*/T2 remains challenging, the effects of controlled and standardized variations in the fraction of inspired oxygen are used for bench marking. This MRI method may be useful for investigating renal blood oxygenation of small rodents in vivo under various experimental (patho)physiological conditions.

Details

Title: Subtitle: Experimental Protocol for MRI Mapping of the Blood Oxygenation-Sensitive Parameters T2 and T2 in the Kidney - Chapter 23
Creators: Andreas Pohlmann - Max Delbrück Center
Kaixuan Zhao - Max Delbrück Center
Sean B Fain - University of Iowa, Radiology
Pottumarthi V Prasad - NorthShore University HealthSystem
Thoralf Niendorf - Max Delbrück Center
Resource Type: Book chapter
Publication Details: Preclinical MRI of the Kidney: Methods and Protocols
Series: Methods in Molecular Biology ; volume 2216
DOI: 10.1007/978-1-0716-0978-1_23
PMID: 33476013
PMCID: PMC9703278
Publisher: Springer US
Language: English
Date published: 2021
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Radiology; Electrical and Computer Engineering; Iowa Neuroscience Institute; Health, Sport, and Human Physiology
Record Identifier: 9984274951802771

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