Book chapter
Generation of Hairpin-Based RNAi Vectors for Biological and Therapeutic Application
Methods in Enzymology, pp.275-296
Elsevier Science & Technology
2012
DOI: 10.1016/B978-0-12-386509-0.00014-4
PMID: 22365779
Abstract
RNA interference (RNAi) is a natural process of gene silencing mediated by small RNAs. Shortly after the discovery of the RNAi mechanism, scientists devised various methods of delivering small interfering RNAs (siRNAs) capable of co-opting the endogenous RNAi machinery and suppressing target gene expression based on sequence complementarity. RNAi has since become a powerful tool to study gene function and is being investigated as a potential therapeutic approach to treat a vast array of human diseases (e.g., cancer, viral infections, and dominant genetic disorders). Among the available RNAi vectors are hairpin-based expression platforms (short-hairpin RNAs and artificial microRNAs) designed to mimic endogenously expressed inhibitory RNAs. These RNAi vectors are capable of achieving long-term potent gene silencing in vitro and in vivo. Here, we describe methods to design and generate these hairpin-based vectors and briefly review considerations for downstream applications.
Details
- Title: Subtitle
- Generation of Hairpin-Based RNAi Vectors for Biological and Therapeutic Application
- Creators
- Ryan L Boudreau - Department of Internal Medicine, University of Iowa, Iowa City, Iowa, USABeverly L Davidson - Department of Internal Medicine, University of Iowa, Iowa City, Iowa, USA
- Resource Type
- Book chapter
- Publication Details
- Methods in Enzymology, pp.275-296
- Publisher
- Elsevier Science & Technology
- DOI
- 10.1016/B978-0-12-386509-0.00014-4
- PMID
- 22365779
- eISSN
- 1557-7988
- ISSN
- 0076-6879
- Language
- English
- Date published
- 2012
- Academic Unit
- Iowa Neuroscience Institute; Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
- Record Identifier
- 9984065392402771
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