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Genetic Engineering in the Ferret
Book chapter

Genetic Engineering in the Ferret

Xingshen Sun, Ziying Yan, Xiaoming Liu, Alicia K Olivier and John F Engelhardt
Biology and Diseases of the Ferret, pp.665-683
John Wiley & Sons, Inc
04/25/2014
DOI: 10.1002/9781118782699.ch28

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Abstract

The domestic ferret (Mustela putorius furo) has become an important animal model in many fields of biomedical research. The development of techniques to genetically engineer domestic ferrets has dramatically expanded the potential of this species to study the molecular basis of human diseases. Genetic engineering in ferrets has been made possible through advances in assisted reproductive technologies and molecular tools capable of efficiently targeting genetic changes to the genome of somatic cells. Efficient assisted reproductive technologies in ferrets require knowledge of reproductive physiology, oocyte maturation, fertilization, and embryonic development in this species. This chapter reviews the area that provides the basis for genetic modeling in ferrets. The generation of knockout, knock‐in, and transgenic ferrets is now feasible. The cystic fibrosis (CF) ferret model is the first demonstration of genetic engineering in ferrets, and this model helps to elucidate the pathogenesis of CF and to develop new mechanistic‐based therapies.

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