Book chapter
Helper-Dependent Adenoviral Vectors and Their Use for Neuroscience Applications
High-Resolution Imaging of Cellular Proteins : Methods and Protocols, Vol.1474, pp.73-90
Methods in molecular biology (Clifton, N.J.), 1474, Humana Press
2016
DOI: 10.1007/978-1-4939-6352-2_5
PMID: 27515075
Abstract
Neuroscience research has been revolutionized by the use of recombinant viral vector technology from the basic, preclinical and clinical levels. Currently, multiple recombinant viral vector types are employed with each having its strengths and weaknesses depending on the proposed application. Helper-dependent adenoviral vectors (HdAd) are emerging as ideal viral vectors that solve a major need in the neuroscience field: (1) expression of transgenes that are too large to be packaged by other viral vectors and (2) rapid onset of transgene expression in the absence of cytotoxicity. Here, we describe the methods for large-scale production of HdAd viral vectors for in vivo use with neurospecific transgene expression.
Details
- Title: Subtitle
- Helper-Dependent Adenoviral Vectors and Their Use for Neuroscience Applications
- Creators
- Mónica S Montesinos - Research Group Molecular Mechanisms of Synaptic Function, Max Planck Florida Institute for Neuroscience, Jupiter, FL, 33458, USARachel Satterfield - Research Group Molecular Mechanisms of Synaptic Function, Max Planck Florida Institute for Neuroscience, Jupiter, FL, 33458, USASamuel M Young Jr - Research Group Molecular Mechanisms of Synaptic Function, Max Planck Florida Institute for Neuroscience, Jupiter, FL, 33458, USA. Sam.Young@mpfi.org
- Resource Type
- Book chapter
- Publication Details
- High-Resolution Imaging of Cellular Proteins : Methods and Protocols, Vol.1474, pp.73-90
- Publisher
- Humana Press; New York, NY
- Series
- Methods in molecular biology (Clifton, N.J.); 1474
- DOI
- 10.1007/978-1-4939-6352-2_5
- PMID
- 27515075
- eISBN
- 149396352X; 9781493963522
- ISSN
- 1064-3745
- eISSN
- 1940-6029
- Grant note
- R01 DC014093 / NIDCD NIH HHS R21 EY023408 / NEI NIH HHS
- Language
- English
- Date published
- 2016
- Academic Unit
- Anatomy and Cell Biology; Iowa Neuroscience Institute; Otolaryngology
- Record Identifier
- 9984025306902771
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