Book chapter
In vivo detection of transition metals and nitrosyl-heme complexes using ex vivo electron paramagnetic resonance spectroscopy
Oxidants and Antioxidants, Vol.196, pp.211-219
Methods in molecular biology (Clifton, N.J.), 196, Humana Press
2002
DOI: 10.1385/1-59259-274-0:211
PMID: 12152201
Abstract
Cellular redox environment is a critical determinant of stress-induced cellular responses and the progression of disease (1). Under normal (nonstress) conditions, the cell maintains a strong reducing environment that favors reductive over highly compartmentalized oxidative biochemistry. Exposure of cellular macromolecules to reactive oxygen (ROS) and reactive nitrogen species (RNS) is tightly controlled. Environmental stress can shift the redox balance away from reductive biochemistry however, promoting transition metal activation, and nonprogrammed oxidative and/or nitrosative reactions. Metalcatalyzed oxidative and nitrosative stress have been implicated in the etiology of numerous clinical disorders including inflammation, ischemia-reperfusion injury, rheumatoid arthritis, and aging
Details
- Title: Subtitle
- In vivo detection of transition metals and nitrosyl-heme complexes using ex vivo electron paramagnetic resonance spectroscopy
- Creators
- David M Hall - Department of Exercise Science, University of Iowa, Iowa City, IA, USAGarry R Buettner
- Resource Type
- Book chapter
- Publication Details
- Oxidants and Antioxidants, Vol.196, pp.211-219
- Publisher
- Humana Press; United States
- Series
- Methods in molecular biology (Clifton, N.J.); 196
- DOI
- 10.1385/1-59259-274-0:211
- PMID
- 12152201
- ISSN
- 1064-3745
- eISSN
- 1940-6029
- Grant note
- AG14687 / NIA NIH HHS CA81090 / NCI NIH HHS AG12350 / NIA NIH HHS
- Language
- English
- Date published
- 2002
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984046805802771
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