Book chapter
Interactions Between Catalytic and Inhibitory Subunits of PDE6
Phosphodiesterase Methods and Protocols, pp.277-287
Methods In Molecular Biology, 307, Humana Press
2005
DOI: 10.1385/1-59259-839-0:277
PMID: 15988070
Abstract
Rod and cone photoreceptor guanosine 3′5′-cyclic-monophosphate phosphodiesterases (PDEs) are classified into the PDE6 family of cyclic nucleotide PDEs. A unique feature of the PDE6 enzymes is the presence of inhibitory γ-subunits (Pγ). The inhibitory interaction between Pγ and the rod PDE6αβ catalytic subunits is critically important for understanding the mechanism of phototransduction. Recent insights into the molecular interface between Pγ and PDE6αβ have been achieved using mutagenesis of Pγ, fluorescence labeling, and crosslinking approaches.
Details
- Title: Subtitle
- Interactions Between Catalytic and Inhibitory Subunits of PDE6
- Creators
- Nikolai O Artemyev - Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City
- Resource Type
- Book chapter
- Publication Details
- Phosphodiesterase Methods and Protocols, pp.277-287
- Publisher
- Humana Press; Totowa, NJ
- Series
- Methods In Molecular Biology; 307
- DOI
- 10.1385/1-59259-839-0:277
- PMID
- 15988070
- eISSN
- 1940-6029
- ISSN
- 1064-3745
- Language
- English
- Date published
- 2005
- Academic Unit
- Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
- Record Identifier
- 9984070123502771
Metrics
11 Record Views