Book chapter
Ionic Currents in Larval Muscles of Drosophila
International Review of Neurobiology, pp.191-220
Elsevier Science & Technology
1999
DOI: 10.1016/S0074-7742(08)60546-2
PMID: 10218160
Abstract
The larval muscle preparation provides an excellent in situ assay system for the studies of ion channels in Drosophila. Larval body wall muscles of Drosophila provide a very useful system for studying K+ and Ca2+ currents. This chapter demonstrates that the function of the native K+ and Ca2+ channels can be analyzed with the advanced biophysical and pharmacological techniques that have been applied to other systems. The physiological and biophysical properties of ion channels can be analyzed from the single channel to the whole cell level, and the functional roles of different classes of ion channels can be directly elucidated in Drosophila muscles because of the available mutants. The chapter discusses the subunit composition of the channels, the genes encoding the subunits, the kinetic properties, and voltage dependency of the currents, pharmacological blockers and activators, and the signal transduction pathways regulating or modulating the channel properties. The physiological aspects of the currents are presented. Muscle currents in adults are briefly summarized for comparison with currents in the larval muscles. The role of different ionic currents in the physiology of muscle fibers is discussed in experiments involving current clamping and membrane potential measurements.
Details
- Title: Subtitle
- Ionic Currents in Larval Muscles of Drosophila
- Creators
- Satpal Singh - Department of Biochemical Pharmacology, SUNY at Buffalo, Buffalo, New York 14260Chun-Fang Wu - Department of Biology, University of Iowa, Iowa City, Iowa 52242
- Resource Type
- Book chapter
- Publication Details
- International Review of Neurobiology, pp.191-220
- Publisher
- Elsevier Science & Technology
- DOI
- 10.1016/S0074-7742(08)60546-2
- PMID
- 10218160
- eISSN
- 2162-5514
- ISSN
- 0074-7742
- Language
- English
- Date published
- 1999
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984070506602771
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