Book chapter
Isolation and Patch-Clamp of Primary Adipocytes: Methods and Protocols
pp.145-150
Methods in Molecular Biology, Humana Press Inc
01/01/2017
DOI: 10.1007/978-1-4939-6820-6_14
PMID: 28244048
Abstract
The patch-clamp technique allows for the study of ion channel activity in the native adipocyte environment to better understand the contributions of ion channels to adipocyte signaling. Here, we describe methods for isolating primary mature adipocytes from both mouse and human white adipose tissues (subcutaneous and visceral). From the same preparation, we describe how to culture and differentiate preadipocytes isolated from the stromal vascular fraction. We then describe in detail patch-clamp methods, including both whole-cell and perforated-patch configurations.
Details
- Title: Subtitle
- Isolation and Patch-Clamp of Primary Adipocytes: Methods and Protocols
- Creators
- Yanhui Zhang - University of IowaDan Tong - Roy J. and Lucille A. Carver College of MedicineAnil Mishra - University of IowaLitao Xie - University of IowaIsaac Samuel - Roy J. and Lucille A. Carver College of MedicineJessica K. Smith - Roy J. and Lucille A. Carver College of MedicineRajan Sah - University of Iowa
- Contributors
- J Wu (Editor)
- Resource Type
- Book chapter
- Publication Details
- pp.145-150
- Publisher
- Humana Press Inc; TOTOWA
- Series
- Methods in Molecular Biology
- DOI
- 10.1007/978-1-4939-6820-6_14
- PMID
- 28244048
- eISSN
- 1940-6029
- ISSN
- 1064-3745
- Number of pages
- 6
- Grant note
- American Heart Association Roy Carver Charitable Trust
- Language
- English
- Date published
- 01/01/2017
- Academic Unit
- Surgery; Internal Medicine
- Record Identifier
- 9984322954802771
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