Book chapter
Isolation of Redox-Active Endosomes (Redoxosomes) and Assessment of NOX Activity
Methods in molecular biology (Clifton, N.J.), pp.461-472
Methods in Molecular Biology, Humana Press Inc
01/01/2019
DOI: 10.1007/978-1-4939-9424-3_27
PMID: 31172489
Abstract
Reactive oxygen species (ROS) convey signals essential for proliferation, maintenance, and senescence of a growing list of cell types. Compartmentalization of these signals is integral to cell viability as well as the signaling pathways ROS direct. Redox-active endosomes (redoxosomes) are formed downstream of several ligand-activated receptors. NADPH oxidase (NOX) is a main component of redoxosomes, which recruits multiple proteins (Rac1, NOX2, p67(phox), SOD1). Isolation of redoxosomes and evaluation of how superoxide (O-2(center dot)) production directs receptor signaling at the level of the endosome have enabled a better understanding of biologic processes controlled by ROS. In this chapter, we will first review the major signaling pathways that utilize redoxosomes and components that control its redox-dependent functions. We will then outline biochemical and biophysical methods for the isolation and characterization of redoxosome properties.
Details
- Title: Subtitle
- Isolation of Redox-Active Endosomes (Redoxosomes) and Assessment of NOX Activity
- Creators
- Weam S. Shahin - Roy J. and Lucille A. Carver College of MedicineJohn F. Engelhardt - Roy J. and Lucille A. Carver College of Medicine
- Contributors
- U G Knaus (Editor)T L Leto (Editor)
- Resource Type
- Book chapter
- Publication Details
- Methods in molecular biology (Clifton, N.J.), pp.461-472
- Publisher
- Humana Press Inc; TOTOWA
- Series
- Methods in Molecular Biology
- DOI
- 10.1007/978-1-4939-9424-3_27
- PMID
- 31172489
- eISSN
- 1940-6029
- ISSN
- 1064-3745
- Number of pages
- 12
- Grant note
- R24 DK096518; P30 DK054759 / NIDDK NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) P30DK054759 / NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK)
- Language
- English
- Date published
- 01/01/2019
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984284334702771
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