Book chapter
Measuring Mitochondrial Shape with ImageJ
Techniques to Investigate Mitochondrial Function in Neurons, pp.31-48
Neuromethods, Springer New York
03/18/2017
DOI: 10.1007/978-1-4939-6890-9_2
Abstract
Mitochondria are shaped by opposing fission (division) and fusion events. Mounting evidence indicates that mitochondrial shape influences numerous aspects of mitochondrial function, including ATP production, Ca2+ buffering, and quality control. Despite the recognized importance of mitochondrial dynamics, the literature is rife with subjective, categorical estimates of mitochondrial morphology, preventing reliable comparison of results between groups. This chapter describes stringent, but easily implemented methods for quantification of mitochondrial shape changes using the open-source software package ImageJ. While we provide examples for analysis of epifluorescence images of cultured primary neurons, these methods are easily generalized to other cell types and imaging techniques.
Details
- Title: Subtitle
- Measuring Mitochondrial Shape with ImageJ
- Creators
- Ronald A Merrill - Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, USAKyle H Flippo - Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, USAStefan Strack - Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, USA
- Resource Type
- Book chapter
- Publication Details
- Techniques to Investigate Mitochondrial Function in Neurons, pp.31-48
- Publisher
- Springer New York; New York, NY
- Series
- Neuromethods
- DOI
- 10.1007/978-1-4939-6890-9_2
- eISSN
- 1940-6045
- ISSN
- 0893-2336
- Language
- English
- Date published
- 03/18/2017
- Academic Unit
- Pathology; Iowa Neuroscience Institute; Neuroscience and Pharmacology
- Record Identifier
- 9984068240402771
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