Book chapter
Methods to Generate Genetically Engineered Mouse Models of Soft Tissue Sarcoma
Mouse Models of Cancer, pp.283-295
Methods in Molecular Biology, Springer New York
01/06/2015
DOI: 10.1007/978-1-4939-2297-0_13
PMCID: PMC5612825
PMID: 25636474
Abstract
We discuss the generation of primary soft tissue sarcomas in mice using the Cre-loxP system to activate conditional mutations in oncogenic Kras and the tumor suppressor p53 (LSL-KrasG12D/+; p53flox/flox). Sarcomas can be generated either by adenoviral delivery of Cre recombinase, activation of transgenic Cre recombinase with tamoxifen, or through transplantation of isolated satellite cells with Cre activation in vitro. Various applications of these models are discussed, including anticancer therapies, metastasis, in vivo imaging, and genetic requirements for tumorigenesis.
Details
- Title: Subtitle
- Methods to Generate Genetically Engineered Mouse Models of Soft Tissue Sarcoma
- Creators
- Rebecca D. Dodd - Duke Medical CenterLeonor Añó - Duke University Medical CenterJordan M. Blum - Duke University Medical CenterZhizhong Li - Duke University Medical CenterDavid Van Mater - Duke University Medical CenterDavid G. Kirsch - Duke University Medical Center
- Resource Type
- Book chapter
- Publication Details
- Mouse Models of Cancer, pp.283-295
- Series
- Methods in Molecular Biology
- DOI
- 10.1007/978-1-4939-2297-0_13
- PMID
- 25636474
- PMCID
- PMC5612825
- NLM abbreviation
- Methods Mol Biol
- eISSN
- 1940-6029
- ISSN
- 1064-3745
- Publisher
- Springer New York; New York, NY
- Language
- English
- Date published
- 01/06/2015
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984359935402771
Metrics
17 Record Views