Book chapter
Mitochondria potential, bax "activation," and programmed cell death
Apoptosis and Cancer, Vol.414, pp.95-108
Methods in molecular biology (Clifton, N.J.), 414, Humana Press
2008
DOI: 10.1007/978-1-59745-339-4_9
PMID: 18175815
Abstract
Since the discovery of the key role of cytochrome C in the activation of caspase 9, intense interest has focused on the role of mitochondria in apoptosis/programmed cell death. Mitochondria undergo two major alterations during apoptosis. The first is the permeabilization of the outer mitochondrial membrane. This event is tightly regulated by members of the Bcl-2 family and involves the conformational change of pro-apoptotic family members such as Bax. Second, the electrochemical gradient that is normally present across the inner mitochondrial membrane is lost (membrane depolarization). This event is sometimes mediated by the permeability transition pore (PTP). The order in which these events occur and whether one causes the other has been hotly debated in the literature. Nonetheless, the majority of reports suggest that mitochondria outer membrane permeabilization (MOMP) precedes membrane depolarization. In this chapter, methods that examine membrane depolarization and the conformational change in Bax are described.
Details
- Title: Subtitle
- Mitochondria potential, bax "activation," and programmed cell death
- Creators
- C Michael Knudson - Department of Pathology, University of Iowa College of Medicine, Iowa City, IA, USANicholas M Brown
- Resource Type
- Book chapter
- Publication Details
- Apoptosis and Cancer, Vol.414, pp.95-108
- Publisher
- Humana Press; United States
- Series
- Methods in molecular biology (Clifton, N.J.); 414
- DOI
- 10.1007/978-1-59745-339-4_9
- PMID
- 18175815
- ISSN
- 1064-3745
- eISSN
- 1940-6029
- Language
- English
- Date published
- 2008
- Academic Unit
- Pathology; Radiation Oncology
- Record Identifier
- 9984047654402771
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