Book chapter
Mutagenesis of Chlamydia trachomatis Using TargeTron: Methods and Protocols
pp.165-184
Methods in Molecular Biology, Humana Press Inc
01/01/2019
DOI: 10.1007/978-1-4939-9694-0_12
PMID: 31385276
Abstract
Chlamydia trachomatis is an important human pathogen that prior to 2011 was largely intractable to genetic manipulation. Here we describe the application of a group II intron, referred to as TargeTron, for site-specific insertional inactivation of target genetic loci in the obligate, intracellular bacteria C. trachomatis. In this chapter, we outline the methods for intron retargeting, chlamydia transformation, and mutant verification. We also outline a method for complementation of TargeTron mutants. Furthermore, we discuss potential pitfalls and alternative strategies for generating mutants with TargeTron technology.
Details
- Title: Subtitle
- Mutagenesis of Chlamydia trachomatis Using TargeTron: Methods and Protocols
- Creators
- Mary M. Weber - Roy J. and Lucille A. Carver College of MedicineRobert Faris - Roy J. and Lucille A. Carver College of Medicine
- Contributors
- Amanda Claire Brown (Editor)
- Resource Type
- Book chapter
- Publication Details
- pp.165-184
- Publisher
- Humana Press Inc; TOTOWA
- Series
- Methods in Molecular Biology
- DOI
- 10.1007/978-1-4939-9694-0_12
- PMID
- 31385276
- eISSN
- 1940-6029
- ISSN
- 1064-3745
- Number of pages
- 20
- Grant note
- University of Iowa Carver College of Medicine Department of Microbiology and Immunology
- Language
- English
- Date published
- 01/01/2019
- Academic Unit
- Molecular Physiology and Biophysics; Microbiology and Immunology
- Record Identifier
- 9984297317702771
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