Overlay and bead assay. Determination of calcium channel subunit interaction domains

V E Scott; C A Gurnett; K P Campbell

doi:10.1385/0-89603-487-9:71

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Book chapter

Overlay and bead assay. Determination of calcium channel subunit interaction domains

V E Scott, C A Gurnett and K P Campbell

Protein Targeting Protocols, pp.71-85

Methods in molecular biology (Clifton, N.J.), 88, Humana Press

1998

DOI: 10.1385/0-89603-487-9:71

PMID: 9664299

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Abstract

Based on electrophysiological and pharmacological properties, voltage-dependent Ca2+ channels are classified as L-, N-, T-, and P/Q- type Ca2+ channels (1,2). The brain N-type Ca2+ channels (3) and the skeletal muscle L-type (4,5) have both been purified and shown to have a similar subunit composition (α1-, α2/δ-, and β-subunits).

Amino Acid Sequence

Recombinant Fusion Proteins - isolation & purification

Calcium Channels - chemistry

Molecular Sequence Data

Protein Binding

Epitopes - chemistry

Recombinant Fusion Proteins - chemistry

Details

Title: Subtitle: Overlay and bead assay. Determination of calcium channel subunit interaction domains
Creators: V E Scott - Howard Hughes Medical Institute, Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, USA
C A Gurnett
K P Campbell
Resource Type: Book chapter
Publication Details: Protein Targeting Protocols, pp.71-85
Publisher: Humana Press; Humana Totowa, NJ
Series: Methods in molecular biology (Clifton, N.J.); 88
DOI: 10.1385/0-89603-487-9:71
PMID: 9664299
ISSN: 1064-3745
eISSN: 1940-6029
Language: English
Date published: 1998
Academic Unit: Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
Record Identifier: 9984068369002771

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