PCNA structure and function: insights from structures of PCNA complexes and post-translationally modified PCNA

Lynne M Dieckman; Bret D Freudenthal; M Todd Washington

doi:10.1007/978-94-007-4572-8_15

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Book chapter

PCNA structure and function: insights from structures of PCNA complexes and post-translationally modified PCNA

Lynne M Dieckman, Bret D Freudenthal and M Todd Washington

The Eukaryotic Replisome: a Guide to Protein Structure and Function, Vol.62, pp.281-299

Sub-cellular biochemistry, 299, Springer

2012

DOI: 10.1007/978-94-007-4572-8_15

PMCID: PMC4429884

PMID: 22918591

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Abstract

Proliferating cell nuclear antigen (PCNA), the eukaryotic DNA sliding clamp, forms a ring-shaped homo-trimer that encircles double-stranded DNA. This protein is best known for its ability to confer high processivity to replicative DNA polymerases. However, it does far more than this, because it forms a mobile platform on the DNA that recruits many of the proteins involved in DNA replication, repair, and recombination to replication forks. X-ray crystal structures of PCNA bound to PCNA-binding proteins have provided insights into how PCNA recognizes its binding partners and recruits them to replication forks. More recently, X-ray crystal structures of ubiquitin-modified and SUMO-modified PCNA have provided insights into how these post-translational modifications alter the specificity of PCNA for some of its binding partners. This article focuses on the insights gained from structural studies of PCNA complexes and post-translationally modified PCNA.

Protein Structure, Tertiary

Humans

Ubiquitin - chemistry

Ubiquitin - metabolism

Crystallography, X-Ray

Structure-Activity Relationship

Sumoylation - physiology

Ubiquitin - genetics

Proliferating Cell Nuclear Antigen - chemistry

SUMO-1 Protein - chemistry

Animals

Proliferating Cell Nuclear Antigen - genetics

DNA Replication - physiology

SUMO-1 Protein - genetics

Protein Structure, Quaternary

SUMO-1 Protein - metabolism

Proliferating Cell Nuclear Antigen - metabolism

Details

Title: Subtitle: PCNA structure and function: insights from structures of PCNA complexes and post-translationally modified PCNA
Creators: Lynne M Dieckman - Department of Biochemistry, University of Iowa College of Medicine, Iowa City, IA, 52242-1109, USA
Bret D Freudenthal
M Todd Washington
Resource Type: Book chapter
Publication Details: The Eukaryotic Replisome: a Guide to Protein Structure and Function, Vol.62, pp.281-299
Series: Sub-cellular biochemistry; 299
DOI: 10.1007/978-94-007-4572-8_15
PMID: 22918591
PMCID: PMC4429884
NLM abbreviation: Subcell Biochem
eISBN: 9789400745728; 9400745729
ISSN: 0306-0225
eISSN: 2542-8810
Publisher: Springer; Dordrecht
Grant note: GM081433 / NIGMS NIH HHS R01 GM081433 / NIGMS NIH HHS
Language: English
Date published: 2012
Academic Unit: Radiation Oncology; Biochemistry and Molecular Biology
Record Identifier: 9984025297702771

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